Acoustic Trauma-Induced Hearing Loss Model
Objective: Evaluate the otoprotective efficacy of D-JNKI-1, a JNK inhibitor peptide, against acoustic trauma-induced permanent hearing loss and hair cell death in guinea pigs
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
Not specified • Not mentioned • Not mentioned • Not mentioned
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Protocol Steps
Organ culture preparation
Prepare organ cultures of neonatal mouse cochlea and adult guinea pig cochleae for experimental exposure
Note: Both neonatal mouse and adult guinea pig cochleae were used in the study
View evidence from paper
“The experimental studies included organ cultures of neonatal mouse cochlea exposed to an ototoxic drug and cochleae of adult guinea pigs”
In vitro ototoxin exposure
Expose organ of Corti explants to neomycin ototoxin to induce hair cell damage
Note: Organ of Corti explants were exposed to neomycin
View evidence from paper
“Treatment of the neomycin-exposed organ of Corti explants with D-JNKI-1 completely prevented hair cell death initiated by this ototoxin”
D-JNKI-1 treatment in vitro
Apply D-JNKI-1 peptide to neomycin-exposed organ of Corti explants to assess otoprotection
Note: Treatment completely prevented hair cell death in vitro
View evidence from paper
“Treatment of the neomycin-exposed organ of Corti explants with D-JNKI-1 completely prevented hair cell death initiated by this ototoxin”
In vivo D-JNKI-1 delivery
Directly apply D-JNKI-1 into the scala tympani of guinea pig cochlea prior to ototoxic drug exposure
Note: Direct application into scala tympani prevented nearly all hair cell death and permanent hearing loss from neomycin
View evidence from paper
“Direct application of D-JNKI-1 into the scala tympani of the guinea pig cochlea prevented nearly all hair cell death and permanent hearing loss induced by neomycin ototoxicity”
Acoustic trauma exposure
Expose guinea pig cochleae to acoustic trauma to induce hearing loss
Note: Acoustic trauma was used as an alternative injury model to ototoxic drug exposure
View evidence from paper
“cochleae of adult guinea pigs that were exposed to either an ototoxic drug or acoustic trauma”
D-JNKI-1 treatment for acoustic trauma
Apply D-JNKI-1 locally to prevent acoustic trauma-induced permanent hearing loss in a dose-dependent manner
Note: Local delivery of D-JNKI-1 prevented acoustic trauma-induced permanent hearing loss in a dose-dependent manner
View evidence from paper
“Local delivery of D-JNKI-1 also prevented acoustic trauma-induced permanent hearing loss in a dose-dependent manner”