Fish Collection and Transport
Objective: Collection and transport of Nothobranchius furzeri fish from ephemeral pans in southern Mozambique to establish wild-derived populations in captivity
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
Not specified • Not specified • Not specified • Not mentioned
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Protocol Steps
Identify collection sites
Sample ephemeral pans in southern Mozambique between the Save and Limpopo Rivers. Restrict sampling to transects corresponding to primary/secondary roads.
Note: Collection points were named using a code containing country name, year of collection, and progressive number. Wild-derived populations analyzed were named: MZM-04/02, MZM-04/03, MZM-04/06, and MZM-04/10
View evidence from paper
“In the last week of March 2004, we sampled ephemeral pans in southern Mozambique between the Save and Limpopo Rivers”
Collect fish using hand-nets
Use fine-meshed hand-nets to collect fish from ephemeral pans and pools with muddy substrate in the vicinity of rivers
Note: Fish were collected from eight habitats total
View evidence from paper
“Fish were collected using fine-meshed hand-nets. All these habitats were pools and pans in the vicinity of rivers with a muddy substrate”
Bag individual fish
Place each collected fish into a separate plastic bag with water from the collection site
Note: One fish per bag
View evidence from paper
“Fish were bagged one per bag”
Perform daily water changes during transport
Change all water in each transport bag daily to maintain water quality during transport to captivity
Note: Duration of transport not specified
View evidence from paper
“all water in the bag was changed every day”
Establish fish in captivity
Transport fish to captive facility and establish populations. Successfully bred fish from four of the eight collection habitats
Note: Four habitats were successfully transported and bred in captivity
View evidence from paper
“Individuals of N. furzeri were collected from eight habitats and individuals from four habitats were successfully transported and bred in captivity”