Fish Collection and Transport
Objective: Collection and transport of Nothobranchius furzeri fish from ephemeral pans in southern Mozambique to establish wild-derived populations in captivity
This is a Fish Collection and Transport protocol using Nothobranchius furzeri as the model organism. The procedure involves 5 procedural steps, 1 equipment items, 2 materials. Extracted from a 2008 paper published in PLoS ONE.
Model and subjects
Nothobranchius furzeri • Wild-derived populations from southern Mozambique • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Identify collection sites • Collect fish using hand-nets • Bag individual fish
Primary readouts
- Successful transport of fish from collection site to captivity
- Successful breeding of transported populations in captivity
- Identification of color morphs and their geographic distribution
- Confirmation of species identity and co-occurrence with other Nothobranchius species
Key equipment and reagents
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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Protocol Steps
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Identify collection sites
Sample ephemeral pans in southern Mozambique between the Save and Limpopo Rivers. Restrict sampling to transects corresponding to primary/secondary roads.
Note: Collection points were named using a code containing country name, year of collection, and progressive number. Wild-derived populations analyzed were named: MZM-04/02, MZM-04/03, MZM-04/06, and MZM-04/10
View evidence from paper
“In the last week of March 2004, we sampled ephemeral pans in southern Mozambique between the Save and Limpopo Rivers”
Collect fish using hand-nets
Use fine-meshed hand-nets to collect fish from ephemeral pans and pools with muddy substrate in the vicinity of rivers
Note: Fish were collected from eight habitats total
View evidence from paper
“Fish were collected using fine-meshed hand-nets. All these habitats were pools and pans in the vicinity of rivers with a muddy substrate”
Bag individual fish
Place each collected fish into a separate plastic bag with water from the collection site
Note: One fish per bag
View evidence from paper
“Fish were bagged one per bag”
Perform daily water changes during transport
Change all water in each transport bag daily to maintain water quality during transport to captivity
Note: Duration of transport not specified
View evidence from paper
“all water in the bag was changed every day”
Establish fish in captivity
Transport fish to captive facility and establish populations. Successfully bred fish from four of the eight collection habitats
Note: Four habitats were successfully transported and bred in captivity
View evidence from paper
“Individuals of N. furzeri were collected from eight habitats and individuals from four habitats were successfully transported and bred in captivity”