Gonadal Regression Assessment
Objective: Assess gonadal regression in animals with transplanted SCN grafts in the absence of light
This is a Gonadal Regression Assessment protocol using hamster as the model organism. The procedure involves 3 procedural steps, 1 materials. Extracted from a 1987 paper published in Journal of Neuroscience.
Model and subjects
hamster • Not specified • unknown • adult • Not specified
Study window
Estimated timing pending
Core workflow
SCN Lesion Creation • SCN Graft Implantation • Gonadal Regression Assessment in Constant Darkness
Primary readouts
- Presence or absence of gonadal regression in constant darkness
- Correlation between graft neuropeptide content and functional restoration
- Presence of retinal input to grafts
- Presence of efferents from graft into host brain
Key equipment and reagents
Verified items
0
Direct vendor links
0
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
SCN Lesion Creation
Create lesions of the hamster hypothalamic suprachiasmatic nucleus to make animals arrhythmic
Note: This creates the baseline arrhythmic condition in recipient animals
View evidence from paper
“overt circadian rhythms are permanently disrupted following lesions of the hamster hypothalamic suprachiasmatic nucleus (SCN)”
SCN Graft Implantation
Implant brain grafts containing fetal SCN tissue into the lesioned adult hamsters
Note: Grafts should contain neuropeptides including VIP, NPY, and vasopressin for successful restoration
View evidence from paper
“implantations of brain grafts containing the fetal SCN reestablish circadian rhythms of locomotor activity in adult hamsters”
Gonadal Regression Assessment in Constant Darkness
Assess whether animals with restored locomotor rhythms show gonadal regression in the absence of light
Note: This is conducted in conditions of constant darkness to test whether the graft restores this SCN function
View evidence from paper
“animals with restored locomotor rhythms did not show gonadal regression in the absence of light”