Gravitropic Recovery Assay
Objective: Assessment of recovery of gravitropic response in yucQ mutant seedlings after 90-degree rotation
Gather these items before starting the experiment. Check off items as you prepare.
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Protocol Steps
Initial seedling growth in first gravity direction
Grow yucQ seedlings vertically on MS agar medium containing auxins in the first gravity direction (1st g)
Note: Seedlings grown under continuous light conditions
View evidence from paper
“yucQ seedlings were grown in the first gravity direction (1st g) on vertical MS agar medium with auxins for 4 ds”
90-degree rotation
Rotate the seedling plates by 90 degrees to establish a new gravity direction (2nd g)
Note: This creates a new gravitropic stimulus
View evidence from paper
“then rotated by 90° (2nd g) and cultured for an additional 24 h”
Recovery period after rotation
Culture the rotated seedlings on MS agar medium with auxins to observe gravitropic recovery
Note: Seedlings grown under continuous light conditions during recovery period
View evidence from paper
“rotated by 90° (2nd g) and cultured for an additional 24 h”