In Vivo Ototoxicity and Hearing Assessment
Objective: Evaluate otoprotective efficacy of D-JNKI-1 peptide inhibitor against aminoglycoside and acoustic trauma-induced auditory hair cell death and hearing loss in adult guinea pig cochlea
This is a In Vivo Ototoxicity and Hearing Assessment protocol using guinea pig as the model organism. The procedure involves 6 procedural steps, 2 equipment items, 2 materials. Extracted from a 2003 paper published in Journal of Neuroscience.
Model and subjects
guinea pig • Not specified • unknown • adult • Not specified
Study window
Estimated timing pending
Core workflow
In vivo neomycin ototoxicity induction • Direct D-JNKI-1 application to scala tympani • Acoustic trauma exposure
Primary readouts
- Hair cell death/survival in organ of Corti
- Permanent hearing loss prevention
- Morphological integrity of organ of Corti
- Physiological function of organ of Corti
Key equipment and reagents
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Protocol Steps
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In vivo neomycin ototoxicity induction
Adult guinea pig cochlea exposed to neomycin ototoxin to induce hair cell damage
Note: This is the ototoxicity model condition
View evidence from paper
“cochleae of adult guinea pigs that were exposed to either an ototoxic drug or acoustic trauma”
Direct D-JNKI-1 application to scala tympani
D-JNKI-1 peptide inhibitor directly applied into the scala tympani of the guinea pig cochlea
Note: Local delivery method for otoprotection
View evidence from paper
“direct application of D-JNKI-1 into the scala tympani of the guinea pig cochlea prevented nearly all hair cell death”
Acoustic trauma exposure
Adult guinea pig cochlea exposed to acoustic trauma to induce noise-induced hearing loss
Note: Alternative injury model to neomycin ototoxicity
View evidence from paper
“cochleae of adult guinea pigs that were exposed to either an ototoxic drug or acoustic trauma”
D-JNKI-1 treatment for acoustic trauma
Local delivery of D-JNKI-1 to prevent acoustic trauma-induced permanent hearing loss
Note: Dose-dependent prevention of hearing loss observed
View evidence from paper
“Local delivery of D-JNKI-1 also prevented acoustic trauma-induced permanent hearing loss in a dose-dependent manner”
Hair cell assessment
Evaluation of hair cell death and morphological integrity in organ of Corti
Note: Outcome measure for otoprotection efficacy
View evidence from paper
“prevented nearly all hair cell death and permanent hearing loss induced by neomycin ototoxicity”
Hearing function assessment
Measurement of hearing loss and physiological function of organ of Corti
Note: Functional outcome measure
View evidence from paper
“prevented nearly all hair cell death and permanent hearing loss induced by neomycin ototoxicity”