Source Paper
Corticolimbic Dopamine Neurotransmission Is Temporally Dissociated from the Cognitive and Locomotor Effects of Phencyclidine
Barbara Adams, Bita Moghaddam
Journal of Neuroscience • 1998
Locomotor Activity Test
Objective: Assessment of hyperlocomotion and motor activity following PCP injection as a predictor of psychotic potential and to examine temporal relationship between corticolimbic dopamine neurotransmission and dopamine-dependent behavioral effects
This is a Locomotor Activity Test protocol using rodent as the model organism. The procedure involves 5 procedural steps, 3 equipment items, 1 materials. Extracted from a 1998 paper published in Journal of Neuroscience.
Model and subjects
rodent • Not specified • unknown • Not specified • Not specified
Study window
~2 hour study window | ~11.7 hours hands-on
Core workflow
PCP Administration • Locomotor Activity Measurement • Microdialysis Sampling
Primary readouts
- Dopamine efflux in prefrontal cortex and nucleus accumbens
- Glutamate efflux in prefrontal cortex and nucleus accumbens
- Locomotor activity levels over time
- Performance on discrete trial delayed alternation working memory task
Key equipment and reagents
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Protocol Steps
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PCP Administration
Administer PCP injection to rodent subjects
Note: Injection time point serves as baseline (0 minutes) for subsequent measurements
View evidence from paper
“PCP increased dopamine and glutamate efflux in the prefrontal cortex and nucleus accumbens”
Locomotor Activity Measurement
Monitor and record locomotor activity following PCP injection
Note: Locomotor activity returned to baseline in less than 2 hours after injection
View evidence from paper
“locomotor activity returned to baseline in <2 hr after injection”
Microdialysis Sampling
Collect samples to measure dopamine and glutamate efflux in prefrontal cortex and nucleus accumbens
Note: Dopamine remained elevated well above baseline at 2.5 hours when experiment was terminated
View evidence from paper
“The increase in dopamine in both regions remained elevated well above baseline 2.5 hr after the injection, at which time the experiment was terminated”
Working Memory Task Testing - Early Timepoint
Assess performance on discrete trial delayed alternation task up to 60 minutes post-injection
Note: Impaired performance evident at this timepoint
View evidence from paper
“impaired performance in a discrete trial delayed alternation task, a rodent working memory task, was only evident up to 60 min after PCP injection”
Working Memory Task Testing - Late Timepoint
Assess performance on discrete trial delayed alternation task at 80 minutes post-injection
Note: No impaired performance observed despite elevated cortical dopamine at 300% of baseline
View evidence from paper
“animals tested 80 min after injection, when cortical dopamine release was elevated at 300% of baseline, did not exhibit impaired performance”