Source Paper
A Specific Herbal Mixture of Lavandula, Cloves, Rosa, Astragalus, and Cornus Mas (LCRAC) Improves Cognitive Function in a Rat Model of Streptozotocin-Induced Mild Cognitive Impairment
Jamali N, Kaeidi A, Hassanshahi J, Memarzadeh MR, Tavakoli T et al.
Adv Biomed Res • 2025
Morris Water Maze
Objective: To evaluate hippocampus-dependent spatial learning and memory
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Protocol Steps
Tank setup
Fill black circular tank with water to 35 cm depth at 22±1°C. Position submerged black platform 2 cm below water surface in center of target quadrant. Place four distinct visual cues on surrounding walls.
View evidence from paper
“filled with water (22 ± 1°C) to a depth of 35 cm”
Training trials
Conduct four trials per day for four consecutive days. Place rats in water facing tank wall from different quadrant each trial. Allow 60 seconds to locate hidden platform.
View evidence from paper
“Over four consecutive days, the rats underwent four trials per day”
Data recording during training
Record escape latency (time to reach platform) and path length (distance traveled) using automated video tracking system.
View evidence from paper
“The latency to reach the platform (escape latency) and the distance traveled (path length) were recorded”
Probe test
Remove hidden platform 24 hours after last training session. Allow rats to swim freely for 60 seconds and measure time spent in target quadrant and swimming speed.
View evidence from paper
“Twenty-four h after the last training session, a spatial probe test was conducted”