Oral Gavage Palmitic Acid Administration
Objective: Assess CNS effects of palmitic acid on PKC-θ localization and hypothalamic insulin resistance via oral gavage administration in rodents
Gather these items before starting the experiment. Check off items as you prepare.
As an Amazon Associate, we earn from qualifying purchases. Product links help support this free resource.
Protocol Steps
Oral gavage administration of palmitic acid
Administer palmitic acid to rodents via oral gavage to assess CNS effects on PKC-θ localization and insulin resistance
Note: This method was used as an alternative to direct infusion to deliver palmitic acid to the CNS
View evidence from paper
“CNS exposure to palmitic acid via direct infusion or by oral gavage increased the localization of PKC-θ to cell membranes in the hypothalamus”
Assessment of PKC-θ subcellular localization
Evaluate PKC-θ localization to cell membranes in hypothalamic regions following palmitic acid exposure
Note: Localization changes were associated with impaired hypothalamic insulin and leptin signaling
View evidence from paper
“CNS exposure to palmitic acid via direct infusion or by oral gavage increased the localization of PKC-θ to cell membranes in the hypothalamus, which was associated with impaired hypothalamic insulin and leptin signaling”
Control treatment with oleic acid
Administer oleic acid (monounsaturated fatty acid) as control to demonstrate specificity of palmitic acid effects
Note: Oleic acid did not increase membrane localization of PKC-θ or induce insulin resistance, confirming specificity
View evidence from paper
“the monounsaturated fatty acid, oleic acid, neither increased membrane localization of PKC-θ nor induced insulin resistance”