Source Paper
A Specific Herbal Mixture of Lavandula, Cloves, Rosa, Astragalus, and Cornus Mas (LCRAC) Improves Cognitive Function in a Rat Model of Streptozotocin-Induced Mild Cognitive Impairment
Jamali N, Kaeidi A, Hassanshahi J, Memarzadeh MR, Tavakoli T et al.
Adv Biomed Res • 2025
Passive Avoidance Test
Objective: Evaluate avoidance learning and memory performance by measuring step-through latency in a passive avoidance task
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Equipment4
Materials1
Not specified • Not specified
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Protocol Steps
Habituation
Place each rat individually in the shuttle box apparatus and allow free exploration of both light and dark compartments
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“each rat was individually placed in the apparatus and allowed to freely explore the two compartments for 5 min”
Training session setup
Place the animal in the light compartment
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“the animal was placed in the light compartment for 1 min”
Door opening and entry
Open the guillotine door and allow the rat to enter the dark compartment
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“Upon opening the guillotine door, the rat was allowed to enter the dark compartment”
Shock delivery
Once rat fully enters dark chamber, close door and deliver electric foot shock through grid floor
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“a mild electric foot shock (0.5 mA, 50 Hz) was delivered through the grid floor for 2 seconds”
Post-shock holding
Keep rat in dark compartment after shock delivery
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“After 20 sec, the rat was returned to its home cage”
Return to home cage
Return rat to home cage after training session
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“the rat was returned to its home cage”
Test session
24 hours after training, place rat in light compartment and measure step-through latency to enter dark compartment
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“Twenty-four h after the training session, the test session was conducted. Each rat was again placed in the light compartment, and the latency to enter the dark compartment (step-through latency) was recorded”