Source Paper
A Specific Herbal Mixture of Lavandula, Cloves, Rosa, Astragalus, and Cornus Mas (LCRAC) Improves Cognitive Function in a Rat Model of Streptozotocin-Induced Mild Cognitive Impairment
Jamali N, Kaeidi A, Hassanshahi J, Memarzadeh MR, Tavakoli T et al.
Adv Biomed Res β’ 2025
Passive Avoidance Test
Objective: Evaluate avoidance learning and memory performance by measuring step-through latency in a passive avoidance task
This is a Passive Avoidance Test protocol using Rats as the model organism. The procedure involves 7 procedural steps, 4 equipment items, 1 materials. Extracted from a 2025 paper published in Adv Biomed Res.
Model and subjects
Rats β’ Wistar β’ Male β’ Not specified β’ 60
Study window
~1 day study window | ~6 minutes hands-on
Core workflow
Habituation β’ Training session setup β’ Door opening and entry
Primary readouts
- Step-through latency (time to enter dark compartment during test session)
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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Protocol Steps
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Habituation
Place each rat individually in the shuttle box apparatus and allow free exploration of both light and dark compartments
View evidence from paper
βeach rat was individually placed in the apparatus and allowed to freely explore the two compartments for 5 minβ
Training session setup
Place the animal in the light compartment
View evidence from paper
βthe animal was placed in the light compartment for 1 minβ
Door opening and entry
Open the guillotine door and allow the rat to enter the dark compartment
View evidence from paper
βUpon opening the guillotine door, the rat was allowed to enter the dark compartmentβ
Shock delivery
Once rat fully enters dark chamber, close door and deliver electric foot shock through grid floor
View evidence from paper
βa mild electric foot shock (0.5 mA, 50 Hz) was delivered through the grid floor for 2 secondsβ
Post-shock holding
Keep rat in dark compartment after shock delivery
View evidence from paper
βAfter 20 sec, the rat was returned to its home cageβ
Return to home cage
Return rat to home cage after training session
View evidence from paper
βthe rat was returned to its home cageβ
Test session
24 hours after training, place rat in light compartment and measure step-through latency to enter dark compartment
View evidence from paper
βTwenty-four h after the training session, the test session was conducted. Each rat was again placed in the light compartment, and the latency to enter the dark compartment (step-through latency) was recordedβ