Source Paper
Stress‐Induced Activation of Prolactin‐NR4A1‐Midkine Axis Exacerbates Skin Inflammation
Li Z, Quan H, Liu W, Chen J, Chu M et al.
Adv Sci (Weinh) • 2025
Patient Health Questionnaire-9 (PHQ-9)
Objective: To assess anxiety and depression levels in psoriasis patients and healthy controls using the Patient Health Questionnaire-9 (PHQ-9)
This is a Patient Health Questionnaire-9 (PHQ-9) protocol using Human as the model organism. The procedure involves 5 procedural steps, 1 materials. Extracted from a 2025 paper published in Adv Sci (Weinh).
Model and subjects
Human • Not applicable • Not specified • Not specified • 450
Study window
Estimated timing pending
Core workflow
Subject recruitment and grouping • PHQ-9 assessment administration • Stress level categorization
Primary readouts
- PHQ-9 stress scores
- Correlation between PHQ-9 and PASI scores
- Comparison of stress levels between psoriasis patients and healthy controls
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
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Subject recruitment and grouping
Recruit psoriasis patients (n=211) and healthy controls (n=239)
View evidence from paper
“psoriasis patients ( n = 211) and healthy controls ( n = 239)”
PHQ-9 assessment administration
Conduct Patient Health Questionnaire-9 assessments on all subjects
View evidence from paper
“we conducted Patient Health Questionnaire‐9 (PHQ‐9) assessments”
Stress level categorization
Divide psoriasis patients into high-stress (PHQ-9 ≥ 7) and low-stress (PHQ-9 < 7) groups
View evidence from paper
“psoriasis patients were divided into a high‐stress group (PHQ‐9 ≥ 7) and a low‐stress group (PHQ‐9 < 7)”
Compare stress scores between groups
Compare PHQ-9 scores between psoriasis patients and healthy controls
View evidence from paper
“Compared to healthy controls (PHQ‐9 mean ± SD: 3.7 ± 3.4), psoriasis patients reported an increased stress perception (PHQ‐9 mean ± SD: 7.8 ± 5.0”
Correlation analysis with disease severity
Analyze correlation between PHQ-9 scores and PASI scores
View evidence from paper
“there was a positive correlation between psoriasis severity (PASI) and PHQ‐9 scores ( R = 0.4655, P < 0.01”