Polygraphic Recording
Objective: Monitor sleep-wake cycle through polygraphic recordings to measure non-rapid eye movement sleep and wakefulness in narcoleptic dogs and genetically modified mice
This is a Polygraphic Recording protocol using Dog (narcoleptic) and Mouse as the model organism. The procedure involves 6 procedural steps, 2 equipment items, 5 materials. Extracted from a 2001 paper published in Journal of Neuroscience.
Model and subjects
Dog (narcoleptic) and Mouse • Not specified for dogs; DAT knock-out mice and wild-type controls implied • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Polygraphic Recording Setup • Baseline Sleep-Wake Monitoring • Drug Administration and Concurrent Recording
Primary readouts
- Non-rapid eye movement (NREM) sleep time
- Wakefulness duration and consolidation
- Extracellular dopamine levels in caudate
- Sleep-wake cycle parameters
Key equipment and reagents
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Protocol Steps
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Polygraphic Recording Setup
Establish polygraphic recording system in narcoleptic dogs to monitor sleep-wake cycle parameters
Note: Recording captures non-rapid eye movement sleep and wakefulness states
View evidence from paper
“polygraphic recordings and caudate microdialysate dopamine measurements in narcoleptic dogs”
Baseline Sleep-Wake Monitoring
Record baseline sleep-wake cycle data prior to drug administration
Note: Establishes control measurements for comparison
View evidence from paper
“polygraphic recordings...revealed that the wake-promoting antinarcoleptic compounds modafinil and amphetamine”
Drug Administration and Concurrent Recording
Administer wake-promoting compounds (modafinil, amphetamine, methamphetamine, GBR12909, or caffeine) while simultaneously recording polygraphic data and measuring dopamine levels
Note: Compounds tested include modafinil, amphetamine, methamphetamine, selective DAT blocker GBR12909, and caffeine
View evidence from paper
“wake-promoting antinarcoleptic compounds modafinil and amphetamine increase extracellular dopamine in a hypocretin receptor 2-independent manner”
Dopamine Measurement
Measure caudate microdialysate dopamine levels during drug administration in narcoleptic dogs
Note: Dopamine measurements conducted concurrently with polygraphic recordings
View evidence from paper
“caudate microdialysate dopamine measurements in narcoleptic dogs revealed that the wake-promoting antinarcoleptic compounds modafinil and amphetamine increase extracellular dopamine”
Mouse Sleep-Wake Analysis
In mice, measure non-rapid eye movement sleep time and wakefulness consolidation in DAT knock-out versus wild-type controls
Note: DAT deletion reduced NREM sleep time and increased wakefulness consolidation independently from locomotor effects
View evidence from paper
“deletion of the dopamine transporter (DAT) gene reduced non-rapid eye movement sleep time and increased wakefulness consolidation independently from locomotor effects”
Drug Response Testing in Mice
Test responsiveness of DAT knock-out mice to wake-promoting compounds (modafinil, methamphetamine, GBR12909, and caffeine)
Note: DAT knock-out mice showed no response to modafinil, methamphetamine, and GBR12909 but were hypersensitive to caffeine
View evidence from paper
“DAT knock-out mice were also unresponsive to the normally robust wake-promoting action of modafinil, methamphetamine, and the selective DAT blocker GBR12909 but were hypersensitive to the wake-promoting effects of caffeine”