Source Paper
Anxiolytic- and antidepressant-like effects of the non-peptide vasopressin V <sub>1b</sub> receptor antagonist, SSR149415, suggest an innovative approach for the treatment of stress-related disorders
Guy Griebel, Jacques Simiand, Claudine Serradeil-Le Gal, Jean Wagnon, Marc Pascal et al.
Proceedings of the National Academy of Sciences • 2002
Punished Drinking Test
Objective: Measure anxiolytic-like effects by assessing approach-avoidance conflict when animals drink water in a punishing context, a classical anxiety model
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
Not specified in provided text • Not specified • Not specified • Not mentioned
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Protocol Steps
Administer test compound
Administer SSR149415 to animals at doses ranging from 1 to 30 mg/kg via intraperitoneal (i.p.) or oral (p.o.) routes
Note: Diazepam used as positive control for comparison
View evidence from paper
“SSR149415 produced anxiolytic-like activity at doses that ranged from 1 to 30 mg/kg (i.p. or p.o.)”
Conduct punished drinking test
Place animals in apparatus and measure drinking behavior in context of punishment to assess approach-avoidance conflict and anxiety-like behavior
Note: This is a classical anxiety model measuring approach-avoidance conflict
View evidence from paper
“punished drinking test”
Measure behavioral outcomes
Quantify drinking behavior and approach-avoidance responses as indicators of anxiolytic-like activity
Note: Magnitude of effects compared to diazepam control
View evidence from paper
“the magnitude of these effects was overall less than that of the benzodiazepine anxiolytic diazepam”