Source Paper
Sex‐specific acceleration of Alzheimer's pathogenesis by chronic sleep‐deprivation
Wear D, Morrone CD, Simpson D, Liu F, Hussaini SA et al.
Alzheimers Dement • 2026
Sleep Disruption
behavioralMus musculustransgenic APP NL‐G‐F/NL‐G‐F knock‐in mice
Objective: To disrupt sleep patterns in transgenic mice using auditory and light stimuli to study the effects on behavior and neurological markers
Materials & Equipment Checklist
5 items
Protocol Steps
1
Week 1 Sleep Disruption
Apply random tones (1000-10,000 Hz at ~100 dB) for 30-60 seconds followed by 30-60 seconds of silence, randomly across 24-hour cycle. Apply constant aversive lights during light cycle only.
View evidence from paper
“random tones ranging in frequency from 1000 to 10,000 Hz at approximately 100 dB playing for 30 to 60 s, followed by a period of silence for 30 to 60 s at a time for week 1”
2
Week 2 Sleep Disruption with Modified Parameters
Alter tone and silence periods to 60-90 seconds to prevent habituation. Continue random timing across 24-hour cycle with aversive lights during light cycle.
View evidence from paper
“During week 2, the lengths of the tones and periods of silence were altered to 60 to 90 s to minimize habituation of the mice to the tones”
3
Maintain Ad Libitum Access
Provide continuous access to water and food throughout the 2-week sleep disruption period.
View evidence from paper
“Mice were given water and chow ad libitum throughout the study”