Source Paper
Sex‐specific acceleration of Alzheimer's pathogenesis by chronic sleep‐deprivation
Wear D, Morrone CD, Simpson D, Liu F, Hussaini SA et al.
Alzheimers Dement • 2026
Sleep Disruption
Objective: To disrupt sleep patterns in transgenic mice using auditory and light stimuli to study the effects on behavior and neurological markers
This is a Sleep Disruption protocol using Mus musculus as the model organism. The procedure involves 3 procedural steps, 2 equipment items, 3 materials. Extracted from a 2026 paper published in Alzheimers Dement.
Model and subjects
Mus musculus • transgenic APP NL‐G‐F/NL‐G‐F knock‐in mice • Male and female • 6 months • 16
Study window
Estimated timing pending
Core workflow
Week 1 Sleep Disruption • Week 2 Sleep Disruption with Modified Parameters • Maintain Ad Libitum Access
Primary readouts
- Sleep patterns and movement tracking
- Recognition memory (Novel Object Recognition test)
- Anxiety-like behavior (Elevated Plus Maze)
- Working memory (Y-maze)
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
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- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Week 1 Sleep Disruption
Apply random tones (1000-10,000 Hz at ~100 dB) for 30-60 seconds followed by 30-60 seconds of silence, randomly across 24-hour cycle. Apply constant aversive lights during light cycle only.
View evidence from paper
“random tones ranging in frequency from 1000 to 10,000 Hz at approximately 100 dB playing for 30 to 60 s, followed by a period of silence for 30 to 60 s at a time for week 1”
Week 2 Sleep Disruption with Modified Parameters
Alter tone and silence periods to 60-90 seconds to prevent habituation. Continue random timing across 24-hour cycle with aversive lights during light cycle.
View evidence from paper
“During week 2, the lengths of the tones and periods of silence were altered to 60 to 90 s to minimize habituation of the mice to the tones”
Maintain Ad Libitum Access
Provide continuous access to water and food throughout the 2-week sleep disruption period.
View evidence from paper
“Mice were given water and chow ad libitum throughout the study”