Treadmill Running Exercise
Objective: To determine whether treadmill running exercise provides neuroprotection against brain insults of different etiology and anatomy, and whether this protection is mediated by circulating insulin-like growth factor I (IGF-I)
This is a Treadmill Running Exercise protocol using rodents (mice and rats) as the model organism. The procedure involves 7 procedural steps, 1 equipment items, 3 materials. Extracted from a 2001 paper published in Journal of Neuroscience.
Model and subjects
rodents (mice and rats) • Not specified • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Treadmill running exercise protocol • Hippocampal insult induction • Brainstem insult induction
Primary readouts
- Recovery of behavioral performance compared with sedentary controls
- Intact spatial memory in hippocampal-injured mice
- Motor coordination in brainstem-damaged animals
- Motor coordination in cerebellum-damaged animals
Key equipment and reagents
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Protocol Steps
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Treadmill running exercise protocol
Laboratory rodents were submitted to treadmill running exercise at a distance of 1 km per day
Note: Exercise was performed either before or after neurotoxin insult, or along the progression of inherited neurodegeneration
View evidence from paper
“Laboratory rodents were submitted to treadmill running (1 km/d) either before or after neurotoxin insult”
Hippocampal insult induction
Domoic acid was administered to induce hippocampal insult in some animals
Note: This was one of three different types of brain insults tested
View evidence from paper
“neurotoxin insult of the hippocampus (domoic acid)”
Brainstem insult induction
3-acetylpyridine was administered to induce brainstem insult in some animals
Note: This was one of three different types of brain insults tested
View evidence from paper
“brainstem (3-acetylpyridine)”
Cerebellar neurodegeneration observation
Animals with inherited neurodegeneration affecting the cerebellum (Purkinje cell degeneration) were observed along progression
Note: This was one of three different types of brain insults tested
View evidence from paper
“inherited neurodegeneration affecting the cerebellum (Purkinje cell degeneration)”
Anti-IGF-I antibody administration
A blocking anti-IGF-I antibody was administered subcutaneously to exercising animals to inhibit exercise-induced brain uptake of IGF-I
Note: This was performed to determine whether neuroprotection by exercise is mediated by IGF-I
View evidence from paper
“subcutaneous administration of a blocking anti-IGF-I antibody to exercising animals to inhibit exercise-induced brain uptake of IGF-I”
Behavioral performance assessment
Animals were assessed for recovery of behavioral performance compared with sedentary controls
Note: Assessments included spatial memory in hippocampal-injured mice and motor coordination in brainstem- and cerebellum-damaged animals
View evidence from paper
“animals show recovery of behavioral performance compared with sedentary ones, i.e., intact spatial memory in hippocampal-injured mice, and normal or near to normal motor coordination”
Neuronal assessment
Neuronal impairment or loss was assessed in all types of injuries
Note: Exercise blocked neuronal impairment or loss in all types of lesions
View evidence from paper
“exercise blocked neuronal impairment or loss in all types of injuries”