Ablation of Unilateral Hippocampal GABAergic Neurons: A Novel Mouse Model of Mesial Temporal Lobe Epilepsy With Hippocampal Sclerosis methods
Aim. Evidence-backed execution summary for Ablation of Unilateral Hippocampal GABAergic Neurons: A Novel Mouse Model of Mesial Temporal Lobe Epilepsy With Hippocampal Sclerosis methods from Ablation of Unilateral Hippocampal GABAergic Neurons: A Novel Mouse Model of Mesial Temporal Lobe Epilepsy With Hippocampal Sclerosis.
Show snapshot details
On this page
This experiment, in seven questions
Jump straight to the part of the recipe you need. Data and provenance labels stay close to the action they support.
Shopping and prep list
What do I need before I start?
mouse
Subject model for the experiment.
- Use
- confirm full cohort details in the source paper
RNAscope In Situ Hybridization
reagent used in the protocol.
- Use
- RNAscope multiplex fluorescent in situ hybridization was performed using the RNAscope Multiplex Fluorescent Reagent Kit v2 (Advanced Cell Diagnostics) according to the manufacturer's protocol. Brifly, fixed-frozen brain sections (20 µm) were fixed in 4% PFA and then dehydrated. Sections were tr...
Fluoro-Jade C (FJC) Staining
reagent used in the protocol.
- Use
- FJC staining was performed to assess neuronal degeneration following interventions according to the manufacturer's protocol (BSS-TR-100-FJT, Biosensis). Briefly, fixed-frozen brain sections (20 µm) were mounted on gelatin-coated slides, dried, and then immersed in a basic alc...
Ablation of GABAergic Neurons Induced Hippocampal MFS
reagent used in the protocol.
- Use
- The anti-synaptoporin antibody visualizes the axons of granule neurons (mossy fibers), reversely from the cell layer to the molecular layer. Hippocampal MFS was classical on the contralateral side of AAV injection in GABAergic neuron ablation models, compared with both the ipsilateral side and the NC models, w...
Methods
Using AAV vectors (flex-DTA or DIO-taCasp3-TEVp), we performed unilateral, partial ablation of GABAergic neurons in the dentate gyrus (DG) or CA1 subregions of VGAT-Cre mice. Spontaneous recurrent seizures (SRS) were monitored by video-EEG. Behavioral tests and histopathological analyse...
- Use
- Using AAV vectors (flex-DTA or DIO-taCasp3-TEVp), we performed unilateral, partial ablation of GABAergic neurons in the dentate gyrus (DG) or CA1 subregions of VGAT-Cre mice. Spontaneous recurrent seizures (SRS) were monitored by video-EEG. Behavioral tests and histopathological analyse...
Intrahippocampal Microinjection and Skull Electrode Implantation
Mice were anesthetized with 250 mg/kg avertin (i.p.) and securely immobilized in a stereotaxic apparatus (RWD, China). Utilizing a microsyringe pump (Nanoject III, Drummond), the AAV vector or KA was administered through a precisely drilled hole in the skull at a slow velocity of 1 nL/s. To accurately ta...
- Use
- Mice were anesthetized with 250 mg/kg avertin (i.p.) and securely immobilized in a stereotaxic apparatus (RWD, China). Utilizing a microsyringe pump (Nanoject III, Drummond), the AAV vector or KA was administered through a precisely drilled hole in the skull at a slow velocity of 1 nL/s. To accurately ta...
Intrahippocampal Microinjection and Skull Electrode Implantation
For mice underwent video electroencephalogram (vEEG) recording, skull electrodes were then inserted and securely fixed using dental cement, following established standards [, ]. The electrodes set up by three stainless steel screws (O.D. = 1 mm, lenth = 2 mm, impedance 700-...
- Use
- For mice underwent video electroencephalogram (vEEG) recording, skull electrodes were then inserted and securely fixed using dental cement, following established standards [, ]. The electrodes set up by three stainless steel screws (O.D. = 1 mm, lenth = 2 mm, impedance 700-...
vEEG Recordings and Analyses
Following the insertion of skull electrodes, mice were connected to the NeuSen H signal acquisition system (Neuracle, Changzhou) for 24-h continuous monitoring, commencing on the 8th day post-surgery and continuing for the subsequent 3 weeks. To assess seizure severity, the revised Racine (r-...
- Use
- Following the insertion of skull electrodes, mice were connected to the NeuSen H signal acquisition system (Neuracle, Changzhou) for 24-h continuous monitoring, commencing on the 8th day post-surgery and continuing for the subsequent 3 weeks. To assess seizure severity, the revised Racine (r-...
Immunofluorescence ( IF ) Staining
Images were acquired utilizing the Mica Microsystem (Leica). Confocal images of z-stack scans (15 µm depth, 1.2 µm step) were generated for mossy fiber staining. The images were imported into ImageJ software for quantitative analysis, according to the standard procedures [, ]. Data colle...
- Use
- Images were acquired utilizing the Mica Microsystem (Leica). Confocal images of z-stack scans (15 µm depth, 1.2 µm step) were generated for mossy fiber staining. The images were imported into ImageJ software for quantitative analysis, according to the standard procedures [, ]. Data colle...
RNAscope In Situ Hybridization
RNAscope multiplex fluorescent in situ hybridization was performed using the RNAscope Multiplex Fluorescent Reagent Kit v2 (Advanced Cell Diagnostics) according to the manufacturer's protocol. Brifly, fixed-frozen brain sections (20 µm) were fixed in 4% PFA and then dehydrated. Sections were tr...
- Use
- RNAscope multiplex fluorescent in situ hybridization was performed using the RNAscope Multiplex Fluorescent Reagent Kit v2 (Advanced Cell Diagnostics) according to the manufacturer's protocol. Brifly, fixed-frozen brain sections (20 µm) were fixed in 4% PFA and then dehydrated. Sections were tr...
Fluoro-Jade C (FJC) Staining
FJC staining was performed to assess neuronal degeneration following interventions according to the manufacturer's protocol (BSS-TR-100-FJT, Biosensis). Briefly, fixed-frozen brain sections (20 µm) were mounted on gelatin-coated slides, dried, and then immersed in a basic alc...
- Use
- FJC staining was performed to assess neuronal degeneration following interventions according to the manufacturer's protocol (BSS-TR-100-FJT, Biosensis). Briefly, fixed-frozen brain sections (20 µm) were mounted on gelatin-coated slides, dried, and then immersed in a basic alc...
Data Analysis
All the experiments were performed in at least six independent biological replicates. EEG filtering analysis was completed under the EEGlab module using Matlab software. Sample sizes (n) represent the number of mice utilized, as detailed in the figure legends. GraphPad Prism 8.0 Software was used for statistical ana...
- Use
- All the experiments were performed in at least six independent biological replicates. EEG filtering analysis was completed under the EEGlab module using Matlab software. Sample sizes (n) represent the number of mice utilized, as detailed in the figure legends. GraphPad Prism 8.0 Software was used for statistical ana...
Data Analysis
Software used for acquisition, scoring, statistics, or reporting.
- Use
- All the experiments were performed in at least six independent biological replicates. EEG filtering analysis was completed under the EEGlab module using Matlab software. Sample sizes (n) represent the number of mice utilized, as detailed in the figure legends. GraphPad Prism 8.0 Software was used for statistical ana...
Before you run
What should be confirmed before execution?
First confirmation
Equipment is listed but no product mappings are linked.
Confirm before execution
This page is backed by a publishable Replication Data Ledger package with zero critical source-verification issues.
Confirm before execution
Open the source paper before finalizing run-specific details.
Procurement checkpoint
Use source-stated vendors where present. Treat mapped products as sourcing options unless the page marks an exact source match.
Open quote workflowStep-by-step procedure
What do I do, in order?
AAV and Reagents
AAV vectors were packaged with titers of 1-10 × 10 12 viral genomes (v.g.)/ml, and 200 nL of these vectors were injected into a single site within the target area of the mouse brain for modeling. Specifically, the rAAV2/9-mCherry-flex-DTA (5.5 × 10 12 v.g./mL, expressing mCherry in all infected cells, simplified as AAV-flex-DTA) was obtained from Addgene plasmid (#58536). The rAAV2/9-DIO-taCasp3-TEVp-mCherry (2.0 × 10 12 v.g./mL, expressing mCherry only in cre-positive cells, simplified as AAV-DIO-taCasp3-TEVp) and the rAAV2/9-DIO-mCherry (2.0 × 10 12 v.g./mL) as negative control (NC) were kind gifts from the Chinese Institute for Brain Research. KA (ab144490) was purchased from Abcam, and 100 nL diluted as 2...
vEEG Recordings and Analyses
Following the insertion of skull electrodes, mice were connected to the NeuSen H signal acquisition system (Neuracle, Changzhou) for 24-h continuous monitoring, commencing on the 8th day post-surgery and continuing for the subsequent 3 weeks. To assess seizure severity, the revised Racine (r-Racine) scale (-1 to 7) was employed, where a tonic-clonic seizure with continuous spike-wave discharges was scored as 5 (when the mouse lay on its belly) or 6 (when it lay on its side or exhibited wild jumping) and was assigned a score of 7 if animal death happened several minutes after seizure onset [ ]. The onset and termination of seizures were precisely determined based on the EEG signal, with the EEG filter settings adjusted to 1-70 Hz and the notch filter activated.
Ablation of Hippocampal GABAergic Neurons by AAV Expressing DTA or taCasp3-TEVp Induced SRS
SRS characteristics induced by hippocampal GABAergic neuron ablation. (A) Timeline of the vEEG recording during 1- and 4-week after modeling. (B) Schematic of the positions where electrodes were inserted in the mouse brains. (C) Representative EEG tracings depicting the interictal period and SRS onset in hippocampal GABAergic neuron ablation models (AAV-mCherry-flex-DTA and AAV-DIO-taCasp3-TEVp-mCherry) and KA injection models. (D) The epileptogenic time course of each group. n = 10 for each group. There is a significant difference in comparison of DTA-CA1 versus DTA-DG ( p < 0.05), DTA-DG versus Casp3-CA1 ( p < 0.001), DTA-DG versus KA- central ( p < 0.01), Casp3-CA1 versus Casp3-DG ( p < 0.01), and Casp3-DG versu...
Ablation of Hippocampal GABAergic Neurons by AAV Expressing DTA or taCasp3-TEVp Induced SRS
The overall effects of the DTA model and the taCasp3 model were comparable, with the latter group showing a seemingly weaker SRS severity, although the difference was not statistically significant ( p > 0.05, Figure ). Regarding the differences among ablation regions, the SRS severity of the CA1 group was generally weaker, characterized by a lower seizure frequency (Figure ), a lower Grade 6 seizure ratio (Figure ), no lethal seizures (Figure ), and a slightly shorter duration (Figure ). Notably, the CA1 model stood out with approximately 50% of mice exhibiting no SRS (Figure ). The overall SRS pattern in the KA model was intermediate compared to each group of hippocampal GABAergic neuron ablation (Figure ). In a word, it was found that GABAergic neuron ablation led to chronic SRS, comparable to KA models.
Measurement outputs
What raw and processed outputs should exist?
Following the insertion of skull electrodes, mice were connected to the NeuSen H signal acquisition system (Neuracle, Changzhou) for 24-h continuous monitoring, commencing...
- Raw artifact
- Field or section images captured from matched samples
- Processed artifact
- Selected representative panels with quantified intensity, counts, or area measurements
- Reported as
- Per-group imaging summaries with representative figures and quantified endpoints
OFT and NORT were designed to evaluate anxiety-like behaviors and recognition memory in animal models, respectively. We used these two behavioral tests here to assess cogn...
- Raw artifact
- Field or section images captured from matched samples
- Processed artifact
- Selected representative panels with quantified intensity, counts, or area measurements
- Reported as
- Per-group imaging summaries with representative figures and quantified endpoints
Images were acquired utilizing the Mica Microsystem (Leica). Confocal images of z-stack scans (15 µm depth, 1.2 µm step) were generated for mossy fib...
- Raw artifact
- Field or section images captured from matched samples
- Processed artifact
- Selected representative panels with quantified intensity, counts, or area measurements
- Reported as
- Per-group imaging summaries with representative figures and quantified endpoints
All the experiments were performed in at least six independent biological replicates. EEG filtering analysis was completed under the EEGlab module using Matlab software. Sample...
- Raw artifact
- Field or section images captured from matched samples
- Processed artifact
- Selected representative panels with quantified intensity, counts, or area measurements
- Reported as
- Per-group imaging summaries with representative figures and quantified endpoints
Analysis plan
How should the outputs become interpretable results?
Acquisition
Capture matched images from the relevant tissue region using the same acquisition settings across samples.
inferred from protocolPreprocessing / cleaning
All the experiments were performed in at least six independent biological replicates.
from paperScoring or quantification
Quantify the primary readouts for this experiment: Following the insertion of skull electrodes, mice were connected to the NeuSen H signal acquisition system (Neuracle, Changzhou) for 24-h continuous monitoring, commencing...; OFT and NORT were designed to evaluate anxiety-like behaviors and recognition memory in animal models, respectively. We used these two behavioral tests here to assess cogn...; Images were acquired utilizing the Mica Microsystem (Leica). Confocal images of z-stack scans (15 µm depth, 1.2 µm step) were generated for mossy fib...; All the experiments were performed in at least six independent biological replicates. EEG filtering analysis was completed under the EEGlab module using Matlab software. Sample....
from paperNormalization
Normalize image-derived measurements against the matched acquisition or segmentation rules before comparing groups.
inferred from protocolStatistical comparison
All the experiments were performed in at least six independent biological replicates. EEG filtering analysis was completed under the EEGlab module using Matlab software. Sample...; Hippocampal GABAergic neuron ablation mice were monitored using 24 h/7 d EEG recordings during the 8th and 28th day after modeling (Figure ), with the schemati...; SRS characteristics induced by hippocampal GABAergic neuron ablation. (A) Timeline of the vEEG recording during 1- and 4-week after modeling. (B) Schematic of the po...; The overall effects of the DTA model and the taCasp3 model were comparable, with the latter group showing a seemingly weaker SRS severity, although the difference was not statis...
from paperReporting output
Report representative outputs alongside summary comparisons for Following the insertion of skull electrodes, mice were connected to the NeuSen H signal acquisition system (Neuracle, Changzhou) for 24-h continuous monitoring, commencing..., OFT and NORT were designed to evaluate anxiety-like behaviors and recognition memory in animal models, respectively. We used these two behavioral tests here to assess cogn..., Images were acquired utilizing the Mica Microsystem (Leica). Confocal images of z-stack scans (15 µm depth, 1.2 µm step) were generated for mossy fib..., All the experiments were performed in at least six independent biological replicates. EEG filtering analysis was completed under the EEGlab module using Matlab software. Sample....
inferred from protocolStructured statistical methods
All the experiments were performed in at least six independent biological replicates. EEG filtering analysis was completed under the EEGlab module using Matlab software. Sample...; Hippocampal GABAergic neuron ablation mice were monitored using 24 h/7 d EEG recordings during the 8th and 28th day after modeling (Figure ), with the schemati...; SRS characteristics induced by hippocampal GABAergic neuron ablation. (A) Timeline of the vEEG recording during 1- and 4-week after modeling. (B) Schematic of the po...; The overall effects of the DTA model and the taCasp3 model were comparable, with the latter group showing a seemingly weaker SRS severity, although the difference was not statis...
source structuredSource and audit
What supports the facts on this page?
Evidence quotes (4)
AAV vectors were packaged with titers of 1-10 × 10 12 viral genomes (v.g.)/ml, and 200 nL of these vectors were injected into a single site within the target area of the mouse brain for modeling. Specifically, the rAAV2/9-mCherry-flex-DTA (5.5 × 10 12 v.g./mL, expressing mCherry in all infected cells, simplified as AAV-flex-DTA) was obtained from Addgene plasmid (#58536). The rAAV2/9-DIO-taCasp3-TEVp-mCherry (2.0 × 10 12 v.g./mL, expressing mCherry only in cre-positive cells, simplified as AAV-DIO-taCasp3-TEVp) and the rAAV2/9-DIO-mCherry (2.0 × 10 12 v.g./mL) as negative control (NC) were kind gifts from the Chinese Institute for Brain Research. KA (ab144490) was purchased from Abcam, and 100 nL diluted as 2 mg/mL in saline was injected per mouse brain.
Following the insertion of skull electrodes, mice were connected to the NeuSen H signal acquisition system (Neuracle, Changzhou) for 24-h continuous monitoring, commencing on the 8th day post-surgery and continuing for the subsequent 3 weeks. To assess seizure severity, the revised Racine (r-Racine) scale (-1 to 7) was employed, where a tonic-clonic seizure with continuous spike-wave discharges was scored as 5 (when the mouse lay on its belly) or 6 (when it lay on its side or exhibited wild jumping) and was assigned a score of 7 if animal death happened several minutes after seizure onset [ ]. The onset and termination of seizures were precisely determined based on the EEG signal, with the EEG filter settings adjusted to 1-70 Hz and the notch filter activated.
SRS characteristics induced by hippocampal GABAergic neuron ablation. (A) Timeline of the vEEG recording during 1- and 4-week after modeling. (B) Schematic of the positions where electrodes were inserted in the mouse brains. (C) Representative EEG tracings depicting the interictal period and SRS onset in hippocampal GABAergic neuron ablation models (AAV-mCherry-flex-DTA and AAV-DIO-taCasp3-TEVp-mCherry) and KA injection models. (D) The epileptogenic time course of each group. n = 10 for each group. There is a significant difference in comparison of DTA-CA1 versus DTA-DG ( p < 0.05), DTA-DG versus Casp3-CA1 ( p < 0.001), DTA-DG versus KA- central ( p < 0.01), Casp3-CA1 versus Casp3-DG ( p < 0.01), and Casp3-DG versus KA- central ( p < 0.01), with no significant difference between the other two groups. (E) Summary of the weekly SRS frequency for each group highlighting the mean SRS rate and differences between groups. n = 6 for NC group, n = 10 for each other group,...
The overall effects of the DTA model and the taCasp3 model were comparable, with the latter group showing a seemingly weaker SRS severity, although the difference was not statistically significant ( p > 0.05, Figure ). Regarding the differences among ablation regions, the SRS severity of the CA1 group was generally weaker, characterized by a lower seizure frequency (Figure ), a lower Grade 6 seizure ratio (Figure ), no lethal seizures (Figure ), and a slightly shorter duration (Figure ). Notably, the CA1 model stood out with approximately 50% of mice exhibiting no SRS (Figure ). The overall SRS pattern in the KA model was intermediate compared to each group of hippocampal GABAergic neuron ablation (Figure ). In a word, it was found that GABAergic neuron ablation led to chronic SRS, comparable to KA models.
Machine-readable layer
[
{
"@context": "https://schema.org",
"@type": "HowTo",
"name": "Ablation of Unilateral Hippocampal GABAergic Neurons: A Novel Mouse Model of Mesial Temporal Lobe Epilepsy With Hippocampal Sclerosis methods",
"description": "Evidence-backed execution summary for Ablation of Unilateral Hippocampal GABAergic Neurons: A Novel Mouse Model of Mesial Temporal Lobe Epilepsy With Hippocampal Sclerosis methods from Ablation of Unilateral Hippocampal GABAergic Neurons: A Novel Mouse Model of Mesial Temporal Lobe Epilepsy With Hippocampal Sclerosis.",
"step": [
{
"@type": "HowToStep",
"position": 1,
"name": "AAV and Reagents",
"text": "AAV vectors were packaged with titers of 1-10 × 10 12 viral genomes (v.g.)/ml, and 200 nL of these vectors were injected into a single site within the target area of the mouse brain for modeling. Specifically, the rAAV2/9-mCherry-flex-DTA (5.5 × 10 12 v.g./mL, expressing mCherry in all infected cells, simplified as AAV-flex-DTA) was obtained from Addgene plasmid (#58536). The rAAV2/9-DIO-taCasp3-TEVp-mCherry (2.0 × 10 12 v.g./mL, expressing mCherry only in cre-positive cells, simplified as AAV-DIO-taCasp3-TEVp) and the rAAV2/9-DIO-mCherry (2.0 × 10 12 v.g./mL) as negative control (NC) were kind gifts from the Chinese Institute for Brain Research. KA (ab144490) was purchased from Abcam, and 100 nL diluted as 2\b..."
},
{
"@type": "HowToStep",
"position": 2,
"name": "vEEG Recordings and Analyses",
"text": "Following the insertion of skull electrodes, mice were connected to the NeuSen H signal acquisition system (Neuracle, Changzhou) for 24-h continuous monitoring, commencing on the 8th day post-surgery and continuing for the subsequent 3 weeks. To assess seizure severity, the revised Racine (r-Racine) scale (-1 to 7) was employed, where a tonic-clonic seizure with continuous spike-wave discharges was scored as 5 (when the mouse lay on its belly) or 6 (when it lay on its side or exhibited wild jumping) and was assigned a score of 7 if animal death happened several minutes after seizure onset [ ]. The onset and termination of seizures were precisely determined based on the EEG signal, with the EEG filter settings adjusted to 1-70 Hz and the notch filter activated."
},
{
"@type": "HowToStep",
"position": 3,
"name": "Ablation of Hippocampal GABAergic Neurons by AAV Expressing DTA or taCasp3-TEVp Induced SRS",
"text": "SRS characteristics induced by hippocampal GABAergic neuron ablation. (A) Timeline of the vEEG recording during 1- and 4-week after modeling. (B) Schematic of the positions where electrodes were inserted in the mouse brains. (C) Representative EEG tracings depicting the interictal period and SRS onset in hippocampal GABAergic neuron ablation models (AAV-mCherry-flex-DTA and AAV-DIO-taCasp3-TEVp-mCherry) and KA injection models. (D) The epileptogenic time course of each group. n = 10 for each group. There is a significant difference in comparison of DTA-CA1 versus DTA-DG ( p < 0.05), DTA-DG versus Casp3-CA1 ( p < 0.001), DTA-DG versus KA- central ( p < 0.01), Casp3-CA1 versus Casp3-DG ( p < 0.01), and Casp3-DG versu..."
},
{
"@type": "HowToStep",
"position": 4,
"name": "Ablation of Hippocampal GABAergic Neurons by AAV Expressing DTA or taCasp3-TEVp Induced SRS",
"text": "The overall effects of the DTA model and the taCasp3 model were comparable, with the latter group showing a seemingly weaker SRS severity, although the difference was not statistically significant ( p > 0.05, Figure ). Regarding the differences among ablation regions, the SRS severity of the CA1 group was generally weaker, characterized by a lower seizure frequency (Figure ), a lower Grade 6 seizure ratio (Figure ), no lethal seizures (Figure ), and a slightly shorter duration (Figure ). Notably, the CA1 model stood out with approximately 50% of mice exhibiting no SRS (Figure ). The overall SRS pattern in the KA model was intermediate compared to each group of hippocampal GABAergic neuron ablation (Figure ). In a word, it was found that GABAergic neuron ablation led to chronic SRS, comparable to KA models."
}
],
"tool": [
{
"@type": "HowToTool",
"name": "Methods"
},
{
"@type": "HowToTool",
"name": "Intrahippocampal Microinjection and Skull Electrode Implantation"
},
{
"@type": "HowToTool",
"name": "Intrahippocampal Microinjection and Skull Electrode Implantation"
},
{
"@type": "HowToTool",
"name": "vEEG Recordings and Analyses"
},
{
"@type": "HowToTool",
"name": "Immunofluorescence ( IF ) Staining"
},
{
"@type": "HowToTool",
"name": "RNAscope In Situ Hybridization"
},
{
"@type": "HowToTool",
"name": "Fluoro-Jade C (FJC) Staining"
},
{
"@type": "HowToTool",
"name": "Data Analysis"
}
],
"supply": [
{
"@type": "HowToSupply",
"name": "RNAscope In Situ Hybridization"
},
{
"@type": "HowToSupply",
"name": "Fluoro-Jade C (FJC) Staining"
},
{
"@type": "HowToSupply",
"name": "Ablation of GABAergic Neurons Induced Hippocampal MFS"
}
],
"isBasedOn": {
"@type": "ScholarlyArticle",
"headline": "Ablation of Unilateral Hippocampal GABAergic Neurons: A Novel Mouse Model of Mesial Temporal Lobe Epilepsy With Hippocampal Sclerosis",
"datePublished": "2026",
"author": [
{
"@type": "Person",
"name": "Ting Tang"
},
{
"@type": "Person",
"name": "Jinkun Xu"
},
{
"@type": "Person",
"name": "Bin Fu"
},
{
"@type": "Person",
"name": "Chao Geng"
},
{
"@type": "Person",
"name": "Yuhao Li"
},
{
"@type": "Person",
"name": "Yuying Qi"
},
{
"@type": "Person",
"name": "Changkai Hou"
},
{
"@type": "Person",
"name": "Junping He"
},
{
"@type": "Person",
"name": "Yihe Wang"
},
{
"@type": "Person",
"name": "Yumin Luo"
},
{
"@type": "Person",
"name": "Guoguang Zhao"
}
],
"identifier": "10.1002/cns.70772"
}
},
{
"@context": "https://schema.org",
"@type": "BreadcrumbList",
"itemListElement": [
{
"@type": "ListItem",
"position": 1,
"name": "Experiments",
"item": "https://replicatescience.com/experiments"
},
{
"@type": "ListItem",
"position": 2,
"name": "Ablation of Unilateral Hippocampal GABAergic Neurons: A Novel Mouse Model of Mesial Temporal Lobe Epilepsy With Hippocampal Sclerosis methods",
"item": "https://replicatescience.com/experiments/ablation-of-unilateral-hippocampal-gabaergic-neurons-a-novel-mouse-model-of-mesial-temporal-lobe-epilepsy-with-hippocampal-sclerosis-methods-ting-tang-pmc12859687/ablation-of-unilateral-hippocampal-gabaergic-neurons-a-novel-mouse-model-of-mesi"
}
]
}
]