Adult Heart Hypertrophy Assessment
Objective: Assessed attenuation of cardiomyocyte hypertrophy by miR-1 in the intact adult heart in vivo
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Materials1
Not specified • Not applicable • Not mentioned • Not mentioned
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Protocol Steps
Assess miR-1 effects on calmodulin expression
Evaluated how cardiomyocyte-restricted microRNA miR-1 inhibited translation of calmodulin-encoding mRNAs via conserved target sites within their 3' untranslated regions
Note: This was the first demonstration that calmodulin levels are regulated posttranscriptionally in heart failure
View evidence from paper
“The cardiomyocyte-restricted microRNA miR-1 inhibited the translation of calmodulin-encoding mRNAs via highly conserved target sites within their 3' untranslated regions”
Assess calcium-calmodulin signaling pathway effects
Evaluated miR-1's effect on downregulating calcium-calmodulin signaling through calcineurin to NFAT pathway
Note: Consistent with calmodulin expression effects
View evidence from paper
“In keeping with its effect on calmodulin expression, miR-1 downregulated calcium-calmodulin signaling through calcineurin to NFAT”
Assess transcription factor regulation
Evaluated miR-1's negative regulation of expression of Mef2a and Gata4, key transcription factors that mediate calcium-dependent changes in gene expression
Note: These are hypertrophy-associated genes
View evidence from paper
“miR-1 also negatively regulated the expression of Mef2a and Gata4, key transcription factors that mediate calcium-dependent changes in gene expression”
Assess cardiomyocyte hypertrophy in cultured cells
Measured attenuation of cardiomyocyte hypertrophy in cultured neonatal rat cardiomyocytes following miR-1 treatment
Note: In vitro model system
View evidence from paper
“miR-1 attenuated cardiomyocyte hypertrophy in cultured neonatal rat cardiomyocytes and in the intact adult heart”
Assess cardiomyocyte hypertrophy in intact adult heart
Measured attenuation of cardiomyocyte hypertrophy in the intact adult heart in vivo following miR-1 intervention
Note: In vivo model system
View evidence from paper
“miR-1 attenuated cardiomyocyte hypertrophy in cultured neonatal rat cardiomyocytes and in the intact adult heart”