Aversive Stimulus Response Test
behavioralNot explicitly stated in provided textNot explicitly stated in provided text
Objective: Assessment of orexin A-mediated synaptic transmission in response to an arousing, aversive stimulus to determine selectivity for positive reinforcement
Protocol Steps
1
Aversive Stimulus Application
Apply an arousing, aversive stimulus to subjects during experimental session
Not explicitly stated in provided textNot explicitly stated in provided text
Note: This is the key manipulation to test selectivity of orexin A-mediated synaptic transmission
View evidence from paper
“oxA/hcrt-1-mediated excitatory synaptic transmission onto VTA neurons was not potentiated following an arousing, aversive stimulus”
2
Measure Synaptic Transmission Response
Measure orexin A/hypocretin-1-mediated glutamatergic synaptic transmission onto ventral tegmental area neurons in response to the aversive stimulus
Not explicitly stated in provided textNot explicitly stated in provided text
Note: Compare response to aversive stimulus versus positive reinforcer conditions
View evidence from paper
“oxA/hcrt-1-mediated glutamatergic synaptic transmission was potentiated selectively with highly salient positive reinforcers”