Aversive Stimulus Response Test
Objective: Assessment of orexin A-mediated synaptic transmission in response to an arousing, aversive stimulus to determine selectivity for positive reinforcement
This is a Aversive Stimulus Response Test protocol using Not explicitly stated in provided text as the model organism. The procedure involves 2 procedural steps. Extracted from a 2009 paper published in Journal of Neuroscience.
Model and subjects
Not explicitly stated in provided text • Not explicitly stated in provided text • unknown • Not explicitly stated in provided text • Not explicitly stated in provided text
Study window
Estimated timing pending
Core workflow
Aversive Stimulus Application • Measure Synaptic Transmission Response
Primary readouts
- Potentiation of orexin A-mediated excitatory synaptic transmission onto VTA neurons
- Selectivity of orexin signaling for positive versus aversive stimuli
- Presynaptic glutamatergic input strength to ventral tegmental area
Key equipment and reagents
Verified items
0
Direct vendor links
0
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Aversive Stimulus Application
Apply an arousing, aversive stimulus to subjects during experimental session
Note: This is the key manipulation to test selectivity of orexin A-mediated synaptic transmission
View evidence from paper
“oxA/hcrt-1-mediated excitatory synaptic transmission onto VTA neurons was not potentiated following an arousing, aversive stimulus”
Measure Synaptic Transmission Response
Measure orexin A/hypocretin-1-mediated glutamatergic synaptic transmission onto ventral tegmental area neurons in response to the aversive stimulus
Note: Compare response to aversive stimulus versus positive reinforcer conditions
View evidence from paper
“oxA/hcrt-1-mediated glutamatergic synaptic transmission was potentiated selectively with highly salient positive reinforcers”