Thermal-Ischemic Lesion
Objective: To examine the role of synchronous neuronal activity in axonal sprouting after thermal-ischemic lesions of sensorimotor cortex, and to determine whether blocking this activity prevents sprouting
This is a Thermal-Ischemic Lesion protocol using Not explicitly stated in provided text as the model organism. The procedure involves 6 procedural steps, 4 equipment items, 1 materials. Extracted from a 2002 paper published in Journal of Neuroscience.
Model and subjects
Not explicitly stated in provided text • Not explicitly stated in provided text • unknown • Not explicitly stated in provided text • Not explicitly stated in provided text
Study window
Estimated timing pending
Core workflow
Induce thermal-ischemic lesion • Record neuronal activity on day 1 post-lesion • Record neuronal activity on days 2-3 post-lesion
Primary readouts
- Patterns of synchronized neuronal activity (frequency ranges and timing)
- Presence and extent of axonal sprouting from contralateral homotypic cortex
- Comparison of sprouting between thermal-ischemic lesions and aspiration lesions
- Effect of TTX infusion on blocking neuronal activity and sprouting
Key equipment and reagents
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Protocol Steps
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Induce thermal-ischemic lesion
Create thermal-ischemic lesions (TCL) in sensorimotor cortex of adult animals
Note: This lesion type induces axonal sprouting and produces specific patterns of neuronal activity
View evidence from paper
“Thermal–ischemic lesions (TCL) of sensorimotor cortex, which induce axonal sprouting, produced two sequential patterns of low-frequency, synchronized neuronal activity”
Record neuronal activity on day 1 post-lesion
Monitor and record synchronized neuronal activity in perilesion cortex on day 1 after thermal-ischemic lesion
Note: Early rhythm of synchronous activity with frequency range 0.2-2 Hz
View evidence from paper
“An early rhythm of synchronous neuronal activity occurred in perilesion cortex on day 1 after lesion, with a frequency range of 0.2–2 Hz”
Record neuronal activity on days 2-3 post-lesion
Monitor and record synchronized neuronal activity across widespread cortical areas on days 2 and 3 after thermal-ischemic lesion
Note: Later pattern of activity with frequency range 0.1-0.4 Hz that synchronizes activity across areas containing cell bodies of sprouting axons
View evidence from paper
“A later pattern of activity occurred on days 2 and 3 after lesion, with a frequency range of 0.1–0.4 Hz. This second rhythm synchronized neuronal activity across widespread areas”
Administer chronic TTX infusion
Infuse tetrodotoxin chronically into the lesion site to block synchronous neuronal activity
Note: TTX blocks patterned neuronal activity and prevents axonal sprouting
View evidence from paper
“Chronic TTX infusion into the lesion site blocked the synchronous neuronal activity after TCL as well as axonal sprouting”
Create control aspiration lesions
Create similarly sized aspiration lesions in control animals for comparison
Note: Aspiration lesions do not induce axonal sprouting and do not produce the same patterns of synchronized neuronal activity
View evidence from paper
“similarly sized aspiration lesions, which do not induce axonal sprouting”
Assess axonal sprouting
Examine and quantify axonal sprouting of contralateral corticostriatal neurons into denervated striatum
Note: Sprouting is correlated with synchronous neuronal activity patterns
View evidence from paper
“robust axonal sprouting of contralateral corticostriatal neurons into the denervated striatum after ischemic cortical lesions”