Age-related changes in behavior in C57BL/6J mice from young adulthood to middle age methods
Aim. Evidence-backed execution summary for Age-related changes in behavior in C57BL/6J mice from young adulthood to middle age methods from Age-related changes in behavior in C57BL/6J mice from young adulthood to middle age.
Show snapshot details
On this page
This experiment, in seven questions
Jump straight to the part of the recipe you need. Data and provenance labels stay close to the action they support.
Shopping and prep list
What do I need before I start?
mouse
Subject model for the experiment.
- Use
- confirm full cohort details in the source paper
Additional files
Correlation between rotarod latency and body weight. Correlations between rotarod latency (s) and body weight (g) in (A) all age groups, (B) 2-3-month-old group, (C) 4-5-months-old group, (D) 6-7-months-old group, and (E) 8-12-months-old group. (F) Correlation between rotarod latency (s) and...
- Use
- Correlation between rotarod latency and body weight. Correlations between rotarod latency (s) and body weight (g) in (A) all age groups, (B) 2-3-month-old group, (C) 4-5-months-old group, (D) 6-7-months-old group, and (E) 8-12-months-old group. (F) Correlation between rotarod latency (s) and...
Decreased locomotor activity and altered anxiety-like behaviors in older C57BL/6J mice
The open field test is widely used to measure locomotor activity and anxiety-like behavior [ ]. In the first 5-min period of the test, there were significant effects of Age on the distance traveled (Fig.; F 3,1451 = 8.51, p < 0.0001) and stereotypic counts (Fig.; F 3,1451 R...
- Use
- The open field test is widely used to measure locomotor activity and anxiety-like behavior [ ]. In the first 5-min period of the test, there were significant effects of Age on the distance traveled (Fig.; F 3,1451 = 8.51, p < 0.0001) and stereotypic counts (Fig.; F 3,1451 R...
Decreased locomotor activity and altered anxiety-like behaviors in older C57BL/6J mice
The light/dark transition test is also commonly used to assess anxiety-like behavior. There were significant effects of Age on the distance traveled in the light and dark chambers (Fig.; the light chamber, F 3,1352 = 11.59, p < 0.0001; the dark chamber, F 3,1352 = 4.46,...
- Use
- The light/dark transition test is also commonly used to assess anxiety-like behavior. There were significant effects of Age on the distance traveled in the light and dark chambers (Fig.; the light chamber, F 3,1352 = 11.59, p < 0.0001; the dark chamber, F 3,1352 = 4.46,...
Decreased locomotor activity and altered anxiety-like behaviors in older C57BL/6J mice
In the elevated plus maze test that has been widely used for assessing anxiety-like behavior, there was a significant effect of Age on distance traveled (Fig.; F 3,1231 = 15.11, p < 0.0001). This effect reached study-wide significance. Subjects in older age groups traveled significan...
- Use
- In the elevated plus maze test that has been widely used for assessing anxiety-like behavior, there was a significant effect of Age on distance traveled (Fig.; F 3,1231 = 15.11, p < 0.0001). This effect reached study-wide significance. Subjects in older age groups traveled significan...
Decreased social contacts in older C57BL/6J mice
The social interaction test, in which two unfamiliar mice are placed together in a novel chamber, is used to assess social behavior. There were significant effects of Age on the distance traveled (Fig.; F 3,546 = 8.48, p < 0.0001), number of contacts (Fig.; F 3,546 =...
- Use
- The social interaction test, in which two unfamiliar mice are placed together in a novel chamber, is used to assess social behavior. There were significant effects of Age on the distance traveled (Fig.; F 3,546 = 8.48, p < 0.0001), number of contacts (Fig.; F 3,546 =...
Impaired spatial learning and memory in older C57BL/6J mice
To examine age-related changes in spatial learning and memory, behavioral data of the Barnes maze test were analyzed for the first, fifth, and ninth block of two trials during the training session. There were no significant effects of Age on the number of errors (Fig. ) in the first or fifth blocks (F 3,349 &#...
- Use
- To examine age-related changes in spatial learning and memory, behavioral data of the Barnes maze test were analyzed for the first, fifth, and ninth block of two trials during the training session. There were no significant effects of Age on the number of errors (Fig. ) in the first or fifth blocks (F 3,349 &#...
Reduced contextual and cued fear memory in older C57BL/6J mice
In the context test, in which mice are exposed to the same conditioning chamber 24 hr after the conditioning session, no significant effects of Age were found for freezing and distance traveled (F 3,510 = 0.20, p = 0.8971; F 3,510 = 1.07, p = 0.3605, respectiv...
- Use
- In the context test, in which mice are exposed to the same conditioning chamber 24 hr after the conditioning session, no significant effects of Age were found for freezing and distance traveled (F 3,510 = 0.20, p = 0.8971; F 3,510 = 1.07, p = 0.3605, respectiv...
Reduced contextual and cued fear memory in older C57BL/6J mice
In the cued test following the context test, in which mice were placed in a different shaped chamber with altered context, subjects in the 8-12-, 6-7-, and 4-5-month-old groups exhibited significantly more freezing and traveled shorter distance than 2-3-month-old subjects during the first 3-m...
- Use
- In the cued test following the context test, in which mice were placed in a different shaped chamber with altered context, subjects in the 8-12-, 6-7-, and 4-5-month-old groups exhibited significantly more freezing and traveled shorter distance than 2-3-month-old subjects during the first 3-m...
Before you run
What should be confirmed before execution?
First confirmation
Equipment is listed but no product mappings are linked.
Confirm before execution
This page is backed by a publishable Replication Data Ledger package with zero critical source-verification issues.
Confirm before execution
Open the source paper before finalizing run-specific details.
Procurement checkpoint
Use source-stated vendors where present. Treat mapped products as sourcing options unless the page marks an exact source match.
Open quote workflowStep-by-step procedure
What do I do, in order?
Decreased locomotor activity and altered anxiety-like behaviors in older C57BL/6J mice
The open field test is widely used to measure locomotor activity and anxiety-like behavior [ ]. In the first 5-min period of the test, there were significant effects of Age on the distance traveled (Fig.; F 3,1451 = 8.51, p < 0.0001) and stereotypic counts (Fig.; F 3,1451 = 8.049, p < 0.0001), which achieved study-wide significance. Subjects in older age groups traveled significantly shorter distances than those in younger age groups (8-12 mo < 4-5 and 2-3 mo, p = 0.0009 and p < 0.0001, respectively; 6-7 mo < 2-3 mo, p = 0.0012). The 6-7- and 4-5-month-old subjects showed significantly more stereotypic counts than 2-3-month-old subjects ( p = 0.0007 and p < 0.0001, respectively). Durin...
Decreased social contacts in older C57BL/6J mice
The social interaction test, in which two unfamiliar mice are placed together in a novel chamber, is used to assess social behavior. There were significant effects of Age on the distance traveled (Fig.; F 3,546 = 8.48, p < 0.0001), number of contacts (Fig.; F 3,546 = 11.71, p < 0.0001), and total duration of active contacts (Fig.; F 3,546 = 8.58, p < 0.0001), all of which reached study-wide significance. Older age subjects traveled significantly shorter distances than subjects in the youngest age group (8-12, 6-7, and 4-5 mo < 2-3 mo, p < 0.0001, p = 0.0043, and p = 0.0055, respectively). The number of contacts in the older age groups was significantly lower than those in the younger age groups (8-12 and 6&#...
Decreased immobility in older C57BL/6J mice
The Porsolt forced swim test, in which a mouse is placed into a cylinder filled with water for 10 min a day for 2 days, is performed to measure immobility time as an index of depression-related behavior. One-way ANOVAs revealed significant effects of Age on immobility time (Fig.: day 1, F 3,1084 = 3.18, p = 0.0234; day 2, F 3,1084 = 5.64, p = 0.0008) and distance traveled (Fig.: day 1, F 3,1084 = 5.51, p = 0.0009; day 2, F 3,1084 = 1.06, p = 0.3671). On day 1, immobility times in older age groups were lower than those in the youngest age group, although these differences did not survive a Bonferroni correction (8-12, 6-7, and 4-5 mo < 2-3 mo, p = 0.0255, p = 0.03, and p = 0.0383, respe...
General health and neurological screen
The general health check and neurological screen was conducted as previously described [ - ]. Body weight and rectal temperature were measured. Neuromuscular strength was assessed using the grip strength and wire hang tests. A grip strength meter (O'Hara & Co., Tokyo, Japan) was used to assess forelimb grip strength. Mice were lifted and held by their tail so that their forepaws could grasp a wire grid. The mice were then gently pulled backward by the tail until they released the grid. The peak force applied by the forelimbs of the mouse was recorded in Newtons (N). Each mouse was tested three times, and the largest value was used for statistical analysis. In the wire hang test, the mouse was placed on a wire mesh that was then inverted, and the latency to fall from the wire was recorded with a 60 s cut-off time.
Social interaction test in a novel environment
Social interaction test [, ] was conducted as previously described [ ]. Two mice of same genotype that were previously housed in different cages, were placed into a box together (40 × 40 × 30 cm; O'Hara & Co., Tokyo, Japan) and were allowed to explore freely for 10 min. Mouse behavior was analyzed automatically using ImageSI software (see Section, "Image analysis"). The total duration of contacts (s), number of contacts, total duration of active contacts (s), mean duration per contact, and total distance traveled (cm) were measured. The active contact was defined as follows: images were captured at three frames per second, and distance traveled between two successive frames was calculated for each mouse. If the two mice contacted each other and the distance traveled by either mouse was 5 cm and more, the behavior was...
Startle response/prepulse inhibition test
A startle reflex measurement system (O'Hara & Co., Tokyo, Japan) was used to measure startle response to a loud noise and prepulse inhibition of the startle response. A test session began by placing a mouse in a plastic cylinder where it was left undisturbed for 10 min. White noise (40 ms) was used as the startle stimulus for all trial types. The startle response was recorded for 400 ms starting with the onset of the startle stimulus. The background noise level was 70 dB. The peak startle amplitude was used as a dependent variable. A test session consisted of six trial types (e.g., two types of startle-stimulus-only trials, and four types for prepulse inhibition trials). The intensity of the startle stimulus was either 110 or 120 dB. The prepulse sound was presented 100 ms before the onset of the startle stimulus, and its intensity was 74 or 78...
Measurement outputs
What raw and processed outputs should exist?
Correlation between rotarod latency and body weight. Correlations between rotarod latency (s) and body weight (g) in (A) all age groups, (B) 2-3-month-old group, (C) 4R...
- Raw artifact
- Per-run gait capture with paw placement, timing, and stride features for each animal
- Processed artifact
- Cleaned gait metrics table and recovery trend summary across timepoints
- Reported as
- Group comparisons of gait indices, stride metrics, or recovery curves
The open field test is widely used to measure locomotor activity and anxiety-like behavior [ ]. In the first 5-min period of the test, there were significant effects of Age on t...
- Raw artifact
- Per-run gait capture with paw placement, timing, and stride features for each animal
- Processed artifact
- Cleaned gait metrics table and recovery trend summary across timepoints
- Reported as
- Group comparisons of gait indices, stride metrics, or recovery curves
The light/dark transition test is also commonly used to assess anxiety-like behavior. There were significant effects of Age on the distance traveled in the light and dark chambe...
- Raw artifact
- Per-run gait capture with paw placement, timing, and stride features for each animal
- Processed artifact
- Cleaned gait metrics table and recovery trend summary across timepoints
- Reported as
- Group comparisons of gait indices, stride metrics, or recovery curves
In the elevated plus maze test that has been widely used for assessing anxiety-like behavior, there was a significant effect of Age on distance traveled (Fig.; F 3,1231 &...
- Raw artifact
- Per-run gait capture with paw placement, timing, and stride features for each animal
- Processed artifact
- Cleaned gait metrics table and recovery trend summary across timepoints
- Reported as
- Group comparisons of gait indices, stride metrics, or recovery curves
Analysis plan
How should the outputs become interpretable results?
Acquisition
Collect raw experimental outputs with enough metadata to preserve sample identity, condition, and timing.
inferred from protocolPreprocessing / cleaning
The open field test is widely used to measure locomotor activity and anxiety-like behavior [ ].
from paperScoring or quantification
Quantify the primary readouts for this experiment: Correlation between rotarod latency and body weight. Correlations between rotarod latency (s) and body weight (g) in (A) all age groups, (B) 2-3-month-old group, (C) 4R...; The open field test is widely used to measure locomotor activity and anxiety-like behavior [ ]. In the first 5-min period of the test, there were significant effects of Age on t...; The light/dark transition test is also commonly used to assess anxiety-like behavior. There were significant effects of Age on the distance traveled in the light and dark chambe...; In the elevated plus maze test that has been widely used for assessing anxiety-like behavior, there was a significant effect of Age on distance traveled (Fig.; F 3,1231 &....
from paperStatistical comparison
The open field test is widely used to measure locomotor activity and anxiety-like behavior [ ]. In the first 5-min period of the test, there were significant effects of Age on t...; The light/dark transition test is also commonly used to assess anxiety-like behavior. There were significant effects of Age on the distance traveled in the light and dark chambe...; In the elevated plus maze test that has been widely used for assessing anxiety-like behavior, there was a significant effect of Age on distance traveled (Fig.; F 3,1231 &...; The social interaction test, in which two unfamiliar mice are placed together in a novel chamber, is used to assess social behavior. There were significant effects of Age on the...
from paperReporting output
Report representative outputs alongside summary comparisons for Correlation between rotarod latency and body weight. Correlations between rotarod latency (s) and body weight (g) in (A) all age groups, (B) 2-3-month-old group, (C) 4R..., The open field test is widely used to measure locomotor activity and anxiety-like behavior [ ]. In the first 5-min period of the test, there were significant effects of Age on t..., The light/dark transition test is also commonly used to assess anxiety-like behavior. There were significant effects of Age on the distance traveled in the light and dark chambe..., In the elevated plus maze test that has been widely used for assessing anxiety-like behavior, there was a significant effect of Age on distance traveled (Fig.; F 3,1231 &....
inferred from protocolStructured statistical methods
The open field test is widely used to measure locomotor activity and anxiety-like behavior [ ]. In the first 5-min period of the test, there were significant effects of Age on t...; The light/dark transition test is also commonly used to assess anxiety-like behavior. There were significant effects of Age on the distance traveled in the light and dark chambe...; In the elevated plus maze test that has been widely used for assessing anxiety-like behavior, there was a significant effect of Age on distance traveled (Fig.; F 3,1231 &...; The social interaction test, in which two unfamiliar mice are placed together in a novel chamber, is used to assess social behavior. There were significant effects of Age on the...
source structuredSource and audit
What supports the facts on this page?
Evidence quotes (6)
The open field test is widely used to measure locomotor activity and anxiety-like behavior [ ]. In the first 5-min period of the test, there were significant effects of Age on the distance traveled (Fig.; F 3,1451 = 8.51, p < 0.0001) and stereotypic counts (Fig.; F 3,1451 = 8.049, p < 0.0001), which achieved study-wide significance. Subjects in older age groups traveled significantly shorter distances than those in younger age groups (8-12 mo < 4-5 and 2-3 mo, p = 0.0009 and p < 0.0001, respectively; 6-7 mo < 2-3 mo, p = 0.0012). The 6-7- and 4-5-month-old subjects showed significantly more stereotypic counts than 2-3-month-old subjects ( p = 0.0007 and p < 0.0001, respectively). During the first 5 min, there were trends toward significant effects of Age on the vertical activity (Fig.; F 3,1451 = 2.25, p = 0.0806) and center time (Fig.; F 3,1451 = 2.38, p = 0.0681). The older age groups tended to exhibit reduc...
The social interaction test, in which two unfamiliar mice are placed together in a novel chamber, is used to assess social behavior. There were significant effects of Age on the distance traveled (Fig.; F 3,546 = 8.48, p < 0.0001), number of contacts (Fig.; F 3,546 = 11.71, p < 0.0001), and total duration of active contacts (Fig.; F 3,546 = 8.58, p < 0.0001), all of which reached study-wide significance. Older age subjects traveled significantly shorter distances than subjects in the youngest age group (8-12, 6-7, and 4-5 mo < 2-3 mo, p < 0.0001, p = 0.0043, and p = 0.0055, respectively). The number of contacts in the older age groups was significantly lower than those in the younger age groups (8-12 and 6-7 mo < 2-3 mo, p = 0.0002 and p < 0.0001, respectively; 8-12 and 6-7 mo < 4-5 mo, p = 0.0079 and p = 0.0017, respectively). Subjects in older age groups showed a significantly shorter duration of...
The Porsolt forced swim test, in which a mouse is placed into a cylinder filled with water for 10 min a day for 2 days, is performed to measure immobility time as an index of depression-related behavior. One-way ANOVAs revealed significant effects of Age on immobility time (Fig.: day 1, F 3,1084 = 3.18, p = 0.0234; day 2, F 3,1084 = 5.64, p = 0.0008) and distance traveled (Fig.: day 1, F 3,1084 = 5.51, p = 0.0009; day 2, F 3,1084 = 1.06, p = 0.3671). On day 1, immobility times in older age groups were lower than those in the youngest age group, although these differences did not survive a Bonferroni correction (8-12, 6-7, and 4-5 mo < 2-3 mo, p = 0.0255, p = 0.03, and p = 0.0383, respectively). Similar results were obtained for immobility time on day 2 (8-12 and 4-5 mo < 2-3 mo, p = 0.0013 and p = 0.0013, respectively). On day 1, the 6-7-month-old subjects swam longer distances than the 4-5- and 2-3-month-o...
The general health check and neurological screen was conducted as previously described [ - ]. Body weight and rectal temperature were measured. Neuromuscular strength was assessed using the grip strength and wire hang tests. A grip strength meter (O'Hara & Co., Tokyo, Japan) was used to assess forelimb grip strength. Mice were lifted and held by their tail so that their forepaws could grasp a wire grid. The mice were then gently pulled backward by the tail until they released the grid. The peak force applied by the forelimbs of the mouse was recorded in Newtons (N). Each mouse was tested three times, and the largest value was used for statistical analysis. In the wire hang test, the mouse was placed on a wire mesh that was then inverted, and the latency to fall from the wire was recorded with a 60 s cut-off time.
Social interaction test [, ] was conducted as previously described [ ]. Two mice of same genotype that were previously housed in different cages, were placed into a box together (40 × 40 × 30 cm; O'Hara & Co., Tokyo, Japan) and were allowed to explore freely for 10 min. Mouse behavior was analyzed automatically using ImageSI software (see Section, "Image analysis"). The total duration of contacts (s), number of contacts, total duration of active contacts (s), mean duration per contact, and total distance traveled (cm) were measured. The active contact was defined as follows: images were captured at three frames per second, and distance traveled between two successive frames was calculated for each mouse. If the two mice contacted each other and the distance traveled by either mouse was 5 cm and more, the behavior was considered an "active contact."
A startle reflex measurement system (O'Hara & Co., Tokyo, Japan) was used to measure startle response to a loud noise and prepulse inhibition of the startle response. A test session began by placing a mouse in a plastic cylinder where it was left undisturbed for 10 min. White noise (40 ms) was used as the startle stimulus for all trial types. The startle response was recorded for 400 ms starting with the onset of the startle stimulus. The background noise level was 70 dB. The peak startle amplitude was used as a dependent variable. A test session consisted of six trial types (e.g., two types of startle-stimulus-only trials, and four types for prepulse inhibition trials). The intensity of the startle stimulus was either 110 or 120 dB. The prepulse sound was presented 100 ms before the onset of the startle stimulus, and its intensity was 74 or 78 dB (20 ms). Four combinations of prepulse and startle stimuli were used (74-110, 78-110, 74-120, and 78-120 dB). Six blocks of the six trial types were presented in a pseudorandom order such that each trial type was presented once within a block. The average inter-...
Machine-readable layer
[
{
"@context": "https://schema.org",
"@type": "HowTo",
"name": "Age-related changes in behavior in C57BL/6J mice from young adulthood to middle age methods",
"description": "Evidence-backed execution summary for Age-related changes in behavior in C57BL/6J mice from young adulthood to middle age methods from Age-related changes in behavior in C57BL/6J mice from young adulthood to middle age.",
"step": [
{
"@type": "HowToStep",
"position": 1,
"name": "Decreased locomotor activity and altered anxiety-like behaviors in older C57BL/6J mice",
"text": "The open field test is widely used to measure locomotor activity and anxiety-like behavior [ ]. In the first 5-min period of the test, there were significant effects of Age on the distance traveled (Fig.; F 3,1451 = 8.51, p < 0.0001) and stereotypic counts (Fig.; F 3,1451 = 8.049, p < 0.0001), which achieved study-wide significance. Subjects in older age groups traveled significantly shorter distances than those in younger age groups (8-12 mo < 4-5 and 2-3 mo, p = 0.0009 and p < 0.0001, respectively; 6-7 mo < 2-3 mo, p = 0.0012). The 6-7- and 4-5-month-old subjects showed significantly more stereotypic counts than 2-3-month-old subjects ( p = 0.0007 and p < 0.0001, respectively). Durin..."
},
{
"@type": "HowToStep",
"position": 2,
"name": "Decreased social contacts in older C57BL/6J mice",
"text": "The social interaction test, in which two unfamiliar mice are placed together in a novel chamber, is used to assess social behavior. There were significant effects of Age on the distance traveled (Fig.; F 3,546 = 8.48, p < 0.0001), number of contacts (Fig.; F 3,546 = 11.71, p < 0.0001), and total duration of active contacts (Fig.; F 3,546 = 8.58, p < 0.0001), all of which reached study-wide significance. Older age subjects traveled significantly shorter distances than subjects in the youngest age group (8-12, 6-7, and 4-5 mo < 2-3 mo, p < 0.0001, p = 0.0043, and p = 0.0055, respectively). The number of contacts in the older age groups was significantly lower than those in the younger age groups (8-12 and 6&#..."
},
{
"@type": "HowToStep",
"position": 3,
"name": "Decreased immobility in older C57BL/6J mice",
"text": "The Porsolt forced swim test, in which a mouse is placed into a cylinder filled with water for 10 min a day for 2 days, is performed to measure immobility time as an index of depression-related behavior. One-way ANOVAs revealed significant effects of Age on immobility time (Fig.: day 1, F 3,1084 = 3.18, p = 0.0234; day 2, F 3,1084 = 5.64, p = 0.0008) and distance traveled (Fig.: day 1, F 3,1084 = 5.51, p = 0.0009; day 2, F 3,1084 = 1.06, p = 0.3671). On day 1, immobility times in older age groups were lower than those in the youngest age group, although these differences did not survive a Bonferroni correction (8-12, 6-7, and 4-5 mo < 2-3 mo, p = 0.0255, p = 0.03, and p = 0.0383, respe..."
},
{
"@type": "HowToStep",
"position": 4,
"name": "General health and neurological screen",
"text": "The general health check and neurological screen was conducted as previously described [ - ]. Body weight and rectal temperature were measured. Neuromuscular strength was assessed using the grip strength and wire hang tests. A grip strength meter (O'Hara & Co., Tokyo, Japan) was used to assess forelimb grip strength. Mice were lifted and held by their tail so that their forepaws could grasp a wire grid. The mice were then gently pulled backward by the tail until they released the grid. The peak force applied by the forelimbs of the mouse was recorded in Newtons (N). Each mouse was tested three times, and the largest value was used for statistical analysis. In the wire hang test, the mouse was placed on a wire mesh that was then inverted, and the latency to fall from the wire was recorded with a 60 s cut-off time."
},
{
"@type": "HowToStep",
"position": 5,
"name": "Social interaction test in a novel environment",
"text": "Social interaction test [, ] was conducted as previously described [ ]. Two mice of same genotype that were previously housed in different cages, were placed into a box together (40 × 40 × 30 cm; O'Hara & Co., Tokyo, Japan) and were allowed to explore freely for 10 min. Mouse behavior was analyzed automatically using ImageSI software (see Section, \"Image analysis\"). The total duration of contacts (s), number of contacts, total duration of active contacts (s), mean duration per contact, and total distance traveled (cm) were measured. The active contact was defined as follows: images were captured at three frames per second, and distance traveled between two successive frames was calculated for each mouse. If the two mice contacted each other and the distance traveled by either mouse was 5 cm and more, the behavior was..."
},
{
"@type": "HowToStep",
"position": 6,
"name": "Startle response/prepulse inhibition test",
"text": "A startle reflex measurement system (O'Hara & Co., Tokyo, Japan) was used to measure startle response to a loud noise and prepulse inhibition of the startle response. A test session began by placing a mouse in a plastic cylinder where it was left undisturbed for 10 min. White noise (40 ms) was used as the startle stimulus for all trial types. The startle response was recorded for 400 ms starting with the onset of the startle stimulus. The background noise level was 70 dB. The peak startle amplitude was used as a dependent variable. A test session consisted of six trial types (e.g., two types of startle-stimulus-only trials, and four types for prepulse inhibition trials). The intensity of the startle stimulus was either 110 or 120 dB. The prepulse sound was presented 100 ms before the onset of the startle stimulus, and its intensity was 74 or 78..."
}
],
"tool": [
{
"@type": "HowToTool",
"name": "Additional files"
},
{
"@type": "HowToTool",
"name": "Decreased locomotor activity and altered anxiety-like behaviors in older C57BL/6J mice"
},
{
"@type": "HowToTool",
"name": "Decreased locomotor activity and altered anxiety-like behaviors in older C57BL/6J mice"
},
{
"@type": "HowToTool",
"name": "Decreased locomotor activity and altered anxiety-like behaviors in older C57BL/6J mice"
},
{
"@type": "HowToTool",
"name": "Decreased social contacts in older C57BL/6J mice"
},
{
"@type": "HowToTool",
"name": "Impaired spatial learning and memory in older C57BL/6J mice"
},
{
"@type": "HowToTool",
"name": "Reduced contextual and cued fear memory in older C57BL/6J mice"
},
{
"@type": "HowToTool",
"name": "Reduced contextual and cued fear memory in older C57BL/6J mice"
}
],
"supply": [],
"isBasedOn": {
"@type": "ScholarlyArticle",
"headline": "Age-related changes in behavior in C57BL/6J mice from young adulthood to middle age",
"datePublished": "2016",
"author": [
{
"@type": "Person",
"name": "Hirotaka Shoji"
},
{
"@type": "Person",
"name": "Keizo Takao"
},
{
"@type": "Person",
"name": "Satoko Hattori"
},
{
"@type": "Person",
"name": "Tsuyoshi Miyakawa"
}
],
"identifier": "10.1186/s13041-016-0191-9)"
}
},
{
"@context": "https://schema.org",
"@type": "BreadcrumbList",
"itemListElement": [
{
"@type": "ListItem",
"position": 1,
"name": "Experiments",
"item": "https://replicatescience.com/experiments"
},
{
"@type": "ListItem",
"position": 2,
"name": "Age-related changes in behavior in C57BL/6J mice from young adulthood to middle age methods",
"item": "https://replicatescience.com/experiments/age-related-changes-in-behavior-in-c57bl-6j-mice-from-young-adulthood-to-middle-age-methods-hirotaka-shoji-pmc4730600/age-related-changes-in-behavior-in-c57bl-6j-mice-from-young-adulthood-to-middle-age-mlpgx0bs"
}
]
}
]