Source Paper
Erythropoietin Regulates the<i>In Vitro</i>and<i>In Vivo</i>Production of Neuronal Progenitors by Mammalian Forebrain Neural Stem Cells
Tetsuro Shingo, S. Todd Sorokan, Takuya Shimazaki, Samuel Weiss
Journal of Neuroscience • 2001
Anti-EPO Antibody Infusion into Lateral Ventricles
Objective: To examine the effects of anti-EPO antibody infusion into adult lateral ventricles on neural stem cell numbers and neurogenesis in the subventricular zone and olfactory bulb
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Equipment1
Not explicitly stated • Not mentioned • Not mentioned • Not mentioned
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Protocol Steps
Prepare anti-EPO antibody solution
Prepare anti-EPO neutralizing antibodies for infusion
Note: Antibodies used to block EPO signaling
View evidence from paper
“Infusion of anti-EPO antibodies had the opposite effect: an increase in the number of NSCs in the subventricular zone”
Infuse anti-EPO antibodies into lateral ventricles
Administer anti-EPO antibodies directly into the adult lateral ventricles
Note: Direct infusion into lateral ventricles of adult animals
View evidence from paper
“Infusion of anti-EPO antibodies had the opposite effect: an increase in the number of NSCs in the subventricular zone and a decrease in the number of newly generated cells migrating to the bulb”
Assess neural stem cell numbers in subventricular zone
Quantify the number of neural stem cells in the subventricular zone following anti-EPO antibody infusion
Note: Anti-EPO antibody infusion resulted in increased NSC numbers
View evidence from paper
“Infusion of anti-EPO antibodies had the opposite effect: an increase in the number of NSCs in the subventricular zone”
Measure newly generated cells migrating to olfactory bulb
Quantify the number of newly generated cells migrating from the subventricular zone to the olfactory bulb
Note: Anti-EPO antibody infusion resulted in decreased migration to the bulb
View evidence from paper
“a decrease in the number of newly generated cells migrating to the bulb”