Antidepressants recruit new neurons to improve stress response regulation methods
Show snapshot details
On this page
This experiment, in seven questions
Jump straight to the part of the recipe you need. Data and provenance labels stay close to the action they support.
Shopping and prep list
What do I need before I start?
Biological model pending
Subject model for the experiment.
- Use
- confirm full cohort details in the source paper
Before you run
What should be confirmed before execution?
First confirmation
Species or subject information is missing.
Confirm before execution
Protocol does not list structured equipment.
Confirm before execution
This page shows passages quoted from the paper. Equipment, materials, times, and temperatures are hidden until the page is rebuilt from the full paper and checked by a reviewer. Check the paper before using this method.
Confirm before execution
Open the source paper before finalizing run-specific details.
Procurement checkpoint
Use source-stated vendors where present. Treat mapped products as sourcing options unless the page marks an exact source match.
Open quote workflowStep-by-step procedure
What do I do, in order?
UCMS
The stress regimen used was previously described and is a variant of the chronic mild stress procedures described by Willner in rats. UCMS mice were repeatedly subjected to various socio-environmental stressors according to an unpredictable schedule for a total period of 6-8 weeks. From the third week of UCMS onward, mice were administered intraperitoneal (ip), once a day, with either vehicle, fluoxetine (20 mg kg -1 per day) or SSR125543 (20 mg kg -1 per day). Treatment was always maintained until the end of the experiments. Body weight and coat state were assessed weekly, as markers of the progression of the UCMS-evoked syndrome.,
Cookie test
This test required a device containing three aligned compartments with the same dimension (20 × 20 × 20 cm). Only the colors of the walls and the floor were different between the compartments (Figure 2a). Mice were first familiarized with a chocolate cookie (Pepito, Lu, France) 4.5 weeks before the first testing. One hour before testing, the regular food was removed from the cage lid. At the time of testing, a small amount (2±1 g) of chocolate cookie (or regular food in a control experiment) was placed at the center of the black compartment. The mouse was initially placed in the white compartment of the apparatus. Each session of the test lasted five minutes. The cookie consumption (# bites) was recorded within the 5 min test period. Four sessions of testing were performed within 10 days (inter-test interval: 2 days).
Dexamethasone (DEX) administration procedures
DEX suppression test: mice were i.p. injected with either DEX-P (0.1 mg kg -1, five mice per group) or saline (0.9% NaCl, five mice per group). All fecalthe boli that were voided between 8 and 10 h after the injection were collected to measure the level of fecal corticosterone metabolites.
Dexamethasone (DEX) administration procedures
Intrahippocampal DEX infusion: the infusion procedure was derived from the method previously described. Mice were stereotaxically and bilaterally implanted with guide cannulas (6 mm long, 0.6 mm outer diameter, 0.36 mm inner diameter). The following coordinates were used for the guide cannula implantation: bregma=-3.08, lateral=±2.3, vertical=-1.4. After 1-week recovery period from the surgery, infusion cannulas (7 mm long, 0.3 mm outer diameter, 0.17 mm inner diameter) were placed and extended 1 mm below the guide cannulas. The animals received bilateral infusions during 1.5 min of either vehicle (0.9% NaCl/0.2% ethanol) or DEX (50 ng in 0.6 µl). In one experiment, the brain was collected 2 h following the infusion to assess DEX-induced Fos changes in downstream brain structures and the PVN (Figur...
Chronic stress disrupts the sensitivity of the granule cell network to novelty/glucocorticoid effects
We then directly tested the ability of the DG granule neurons to react to glucocorticoids. These cells can be activated and express immediate early genes when individuals are exposed to a new environment.,, We took advantage of this property to examine the sensitivity of new and older granule cells to a new environment exposure following a single injection of DEX-P or vehicle ( ). For this purpose, we concurrently labeled cells for the neuronal marker NeuN, the immediate early gene product Fos ('activated' granule cells) and the proliferation marker BrdU (4 week-old newborn cells) ( ).
Measurement outputs
What raw and processed outputs should exist?
Analysis plan
How should the outputs become interpretable results?
Acquisition
Collect raw experimental outputs with enough metadata to preserve sample identity, condition, and timing.
inferred from protocolPreprocessing / cleaning
Review raw outputs for quality, remove unusable captures, and organize the data into a comparison-ready table or image set.
inferred from protocolScoring or quantification
Quantify the primary endpoint described in the methods before moving into any statistical comparison.
inferred from protocolStatistical comparison
Statistical method not yet structured for this page.
inferred from protocolReporting output
Report representative outputs alongside the summary comparison used to support the conclusion.
inferred from protocolSource and audit
What supports the facts on this page?
Evidence quotes (5)
The stress regimen used was previously described and is a variant of the chronic mild stress procedures described by Willner in rats. UCMS mice were repeatedly subjected to various socio-environmental stressors according to an unpredictable schedule for a total period of 6-8 weeks. From the third week of UCMS onward, mice were administered intraperitoneal (ip), once a day, with either vehicle, fluoxetine (20 mg kg -1 per day) or SSR125543 (20 mg kg -1 per day). Treatment was always maintained until the end of the experiments. Body weight and coat state were assessed weekly, as markers of the progression of the UCMS-evoked syndrome.,
This test required a device containing three aligned compartments with the same dimension (20 × 20 × 20 cm). Only the colors of the walls and the floor were different between the compartments (Figure 2a). Mice were first familiarized with a chocolate cookie (Pepito, Lu, France) 4.5 weeks before the first testing. One hour before testing, the regular food was removed from the cage lid. At the time of testing, a small amount (2±1 g) of chocolate cookie (or regular food in a control experiment) was placed at the center of the black compartment. The mouse was initially placed in the white compartment of the apparatus. Each session of the test lasted five minutes. The cookie consumption (# bites) was recorded within the 5 min test period. Four sessions of testing were performed within 10 days (inter-test interval: 2 days).
DEX suppression test: mice were i.p. injected with either DEX-P (0.1 mg kg -1, five mice per group) or saline (0.9% NaCl, five mice per group). All fecalthe boli that were voided between 8 and 10 h after the injection were collected to measure the level of fecal corticosterone metabolites.
Intrahippocampal DEX infusion: the infusion procedure was derived from the method previously described. Mice were stereotaxically and bilaterally implanted with guide cannulas (6 mm long, 0.6 mm outer diameter, 0.36 mm inner diameter). The following coordinates were used for the guide cannula implantation: bregma=-3.08, lateral=±2.3, vertical=-1.4. After 1-week recovery period from the surgery, infusion cannulas (7 mm long, 0.3 mm outer diameter, 0.17 mm inner diameter) were placed and extended 1 mm below the guide cannulas. The animals received bilateral infusions during 1.5 min of either vehicle (0.9% NaCl/0.2% ethanol) or DEX (50 ng in 0.6 µl). In one experiment, the brain was collected 2 h following the infusion to assess DEX-induced Fos changes in downstream brain structures and the PVN (Figure 3). In another experiment, the animals were killed by CO 2 asphyxiation 4 h following infusion and trunk blood was collected to measure plasma corticosterone levels (Figure 6).
We then directly tested the ability of the DG granule neurons to react to glucocorticoids. These cells can be activated and express immediate early genes when individuals are exposed to a new environment.,, We took advantage of this property to examine the sensitivity of new and older granule cells to a new environment exposure following a single injection of DEX-P or vehicle ( ). For this purpose, we concurrently labeled cells for the neuronal marker NeuN, the immediate early gene product Fos ('activated' granule cells) and the proliferation marker BrdU (4 week-old newborn cells) ( ).
Machine-readable layer
[
{
"@context": "https://schema.org",
"@type": "HowTo",
"name": "Antidepressants recruit new neurons to improve stress response regulation methods",
"description": "Protocol for Antidepressants recruit new neurons to improve stress response regulation methods",
"step": [
{
"@type": "HowToStep",
"position": 1,
"name": "UCMS",
"text": "The stress regimen used was previously described and is a variant of the chronic mild stress procedures described by Willner in rats. UCMS mice were repeatedly subjected to various socio-environmental stressors according to an unpredictable schedule for a total period of 6-8 weeks. From the third week of UCMS onward, mice were administered intraperitoneal (ip), once a day, with either vehicle, fluoxetine (20 mg kg -1 per day) or SSR125543 (20 mg kg -1 per day). Treatment was always maintained until the end of the experiments. Body weight and coat state were assessed weekly, as markers of the progression of the UCMS-evoked syndrome.,"
},
{
"@type": "HowToStep",
"position": 2,
"name": "Cookie test",
"text": "This test required a device containing three aligned compartments with the same dimension (20 × 20 × 20 cm). Only the colors of the walls and the floor were different between the compartments (Figure 2a). Mice were first familiarized with a chocolate cookie (Pepito, Lu, France) 4.5 weeks before the first testing. One hour before testing, the regular food was removed from the cage lid. At the time of testing, a small amount (2±1 g) of chocolate cookie (or regular food in a control experiment) was placed at the center of the black compartment. The mouse was initially placed in the white compartment of the apparatus. Each session of the test lasted five minutes. The cookie consumption (# bites) was recorded within the 5 min test period. Four sessions of testing were performed within 10 days (inter-test interval: 2 days)."
},
{
"@type": "HowToStep",
"position": 3,
"name": "Dexamethasone (DEX) administration procedures",
"text": "DEX suppression test: mice were i.p. injected with either DEX-P (0.1 mg kg -1, five mice per group) or saline (0.9% NaCl, five mice per group). All fecalthe boli that were voided between 8 and 10 h after the injection were collected to measure the level of fecal corticosterone metabolites."
},
{
"@type": "HowToStep",
"position": 4,
"name": "Dexamethasone (DEX) administration procedures",
"text": "Intrahippocampal DEX infusion: the infusion procedure was derived from the method previously described. Mice were stereotaxically and bilaterally implanted with guide cannulas (6 mm long, 0.6 mm outer diameter, 0.36 mm inner diameter). The following coordinates were used for the guide cannula implantation: bregma=-3.08, lateral=±2.3, vertical=-1.4. After 1-week recovery period from the surgery, infusion cannulas (7 mm long, 0.3 mm outer diameter, 0.17 mm inner diameter) were placed and extended 1 mm below the guide cannulas. The animals received bilateral infusions during 1.5 min of either vehicle (0.9% NaCl/0.2% ethanol) or DEX (50 ng in 0.6 µl). In one experiment, the brain was collected 2 h following the infusion to assess DEX-induced Fos changes in downstream brain structures and the PVN (Figur..."
},
{
"@type": "HowToStep",
"position": 5,
"name": "Chronic stress disrupts the sensitivity of the granule cell network to novelty/glucocorticoid effects",
"text": "We then directly tested the ability of the DG granule neurons to react to glucocorticoids. These cells can be activated and express immediate early genes when individuals are exposed to a new environment.,, We took advantage of this property to examine the sensitivity of new and older granule cells to a new environment exposure following a single injection of DEX-P or vehicle ( ). For this purpose, we concurrently labeled cells for the neuronal marker NeuN, the immediate early gene product Fos ('activated' granule cells) and the proliferation marker BrdU (4 week-old newborn cells) ( )."
}
],
"tool": [],
"supply": [],
"isBasedOn": {
"@type": "ScholarlyArticle",
"headline": "Antidepressants recruit new neurons to improve stress response regulation",
"datePublished": "2011",
"author": [
{
"@type": "Person",
"name": "A Surget"
},
{
"@type": "Person",
"name": "A Tanti"
},
{
"@type": "Person",
"name": "E D Leonardo"
},
{
"@type": "Person",
"name": "A Laugeray"
},
{
"@type": "Person",
"name": "Q Rainer"
},
{
"@type": "Person",
"name": "C Touma"
},
{
"@type": "Person",
"name": "R Palme"
},
{
"@type": "Person",
"name": "G Griebel"
},
{
"@type": "Person",
"name": "Y Ibarguen-Vargas"
},
{
"@type": "Person",
"name": "R Hen"
},
{
"@type": "Person",
"name": "C Belzung"
}
],
"identifier": "10.1038/mp.2011.48"
}
},
{
"@context": "https://schema.org",
"@type": "BreadcrumbList",
"itemListElement": [
{
"@type": "ListItem",
"position": 1,
"name": "Experiments",
"item": "https://replicatescience.com/experiments"
},
{
"@type": "ListItem",
"position": 2,
"name": "Antidepressants recruit new neurons to improve stress response regulation methods",
"item": "https://replicatescience.com/experiments/antidepressants-recruit-new-neurons-to-improve-stress-response-regulation-methods-a-surget-pmc3223314/antidepressants-recruit-new-neurons-to-improve-stress-response-regulation-mlph0ih5"
}
]
}
]