Source Paper
High‐mobility Group Protein 1/ Receptor for Advanced Glycation End Products/ Nuclear Factor‐κB Signalling Pathway Contributes to the Pathogenic Process of Striatal Neuron Impairment in the Rat Model of Parkinson's Disease
Zhu Y, Du Z, Sun L, Huang Z, Jia L et al.
Brain Behav • 2026
Apomorphine-induced Rotation Test
Objective: To assess rotational behavior in rats with 6-OHDA-induced Parkinson's disease model using apomorphine-induced rotation as a measure of dopaminergic denervation
This is a Apomorphine-induced Rotation Test protocol using Rat as the model organism. The procedure involves 5 procedural steps, 1 equipment items, 3 materials. Extracted from a 2026 paper published in Brain Behav.
Model and subjects
Rat • Sprague-Dawley (SD) • Not specified • 8 weeks • 50
Study window
Estimated timing pending
Core workflow
Establish PD model with 6-OHDA injection • Wait for lesion development • Administer apomorphine
Primary readouts
- Number of contralateral 360° rotations in 30 minutes
- Total rotation count (inclusion criterion: >210 rotations)
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Establish PD model with 6-OHDA injection
Inject 8 µL of 6-OHDA solution (2 µg/µL with 0.01% ascorbic acid) into the right middle forebrain bundle using specific coordinates
View evidence from paper
“Each rat was injected with 8 µL of this solution into the right middle forebrain bundle (MFB)”
Wait for lesion development
Allow three weeks following the development of 6-OHDA lesions before testing
View evidence from paper
“over the three weeks following the development of 6‐OHDA lesions”
Administer apomorphine
Give subcutaneous injection of apomorphine at 0.25 mg/kg dose
View evidence from paper
“Apomorphine (0.25 mg/kg, APO; catalog no. 2073/50, Tocris) was administered subcutaneously to the rats”
Count rotations
Count the number of contralateral 360° rotations within a 30-minute period
View evidence from paper
“we counted the number of contralateral 360° rotations within a 30‐min period”
Apply inclusion criteria
Include only rats that completed more than 210 total rotations for further analysis
View evidence from paper
“For further analysis, only rats that completed more than 210 total rotations were included”