Experiments/Central Administration of Dynorphin A

Source Paper

Neurokinin B and Dynorphin A in Kisspeptin Neurons of the Arcuate Nucleus Participate in Generation of Periodic Oscillation of Neural Activity Driving Pulsatile Gonadotropin-Releasing Hormone Secretion in the Goat

Yoshihiro Wakabayashi, Tomoaki Nakada, Ken Murata, Satoshi Ohkura, Kazutaka Mogi et al.

Journal of Neuroscience • 2010

DOI PMC

Central Administration of Dynorphin A

behavioralgoatnot specified

Objective: Investigate the effects of centrally administered dynorphin A on multiple-unit activity volleys and pulsatile luteinizing hormone secretion in the medial basal hypothalamus

This is a Central Administration of Dynorphin A protocol using goat as the model organism. The procedure involves 5 procedural steps, 1 equipment items, 2 materials. Extracted from a 2010 paper published in Journal of Neuroscience.

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Replication Essentials

Model and subjects

goat • not specified • female • not specified • not specified

Study window

Estimated timing pending

Core workflow

Animal preparation • Multiple-unit activity recording • Central administration of dynorphin A

Primary readouts

Multiple-unit activity (MUA) volleys in the medial basal hypothalamus
Pulsatile luteinizing hormone (LH) secretion
Frequency of MUA volleys
Association between MUA bursts and discrete LH pulses

Key equipment and reagents

Electrophysiological recording apparatusDynorphin A (Dyn)

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Protocol Steps

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Animal preparation

Use ovariectomized goats as experimental subjects

not specifiednot specified

Note: Ovariectomized animals were used to establish baseline conditions for recording MUA volleys

View evidence from paper

“bursts of MUA occurred at regular intervals in ovariectomized animals”

Multiple-unit activity recording

Record multiple-unit activity (MUA) in the medial basal hypothalamus using electrophysiological techniques

not specifiednot specified

Note: MUA volleys occurred at regular intervals and were associated with discrete pulses of luteinizing hormone

View evidence from paper

“bursts of MUA occurred at regular intervals in ovariectomized animals and that these repetitive bursts (volleys) were invariably associated with discrete pulses of luteinizing hormone (LH)”