Source Paper
Neurokinin B and Dynorphin A in Kisspeptin Neurons of the Arcuate Nucleus Participate in Generation of Periodic Oscillation of Neural Activity Driving Pulsatile Gonadotropin-Releasing Hormone Secretion in the Goat
Yoshihiro Wakabayashi, Tomoaki Nakada, Ken Murata, Satoshi Ohkura, Kazutaka Mogi et al.
Journal of Neuroscience • 2010
Central Administration of Dynorphin A
Objective: Investigate the effects of centrally administered dynorphin A on multiple-unit activity volleys and pulsatile luteinizing hormone secretion in the medial basal hypothalamus
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Equipment1
not specified • not specified • not specified • not specified
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Protocol Steps
Animal preparation
Use ovariectomized goats as experimental subjects
Note: Ovariectomized animals were used to establish baseline conditions for recording MUA volleys
View evidence from paper
“bursts of MUA occurred at regular intervals in ovariectomized animals”
Multiple-unit activity recording
Record multiple-unit activity (MUA) in the medial basal hypothalamus using electrophysiological techniques
Note: MUA volleys occurred at regular intervals and were associated with discrete pulses of luteinizing hormone
View evidence from paper
“bursts of MUA occurred at regular intervals in ovariectomized animals and that these repetitive bursts (volleys) were invariably associated with discrete pulses of luteinizing hormone (LH)”
Central administration of dynorphin A
Administer dynorphin A centrally and observe effects on MUA volleys and pulsatile LH secretion
Note: Central administration of dynorphin A inhibited MUA volleys and pulsatile LH secretion
View evidence from paper
“central administration of Dyn-inhibit MUA volleys and pulsatile LH secretion”
Central administration of neurokinin B
Administer neurokinin B centrally and observe effects on MUA volleys
Note: NKB induced MUA volleys, contrasting with the inhibitory effect of dynorphin A
View evidence from paper
“NKB induced MUA volleys”
Measurement of gonadal steroid effects
Evaluate the frequency of MUA volleys in relation to gonadal steroid levels
Note: Frequency of MUA volleys was reduced by gonadal steroids, suggesting volleys reflect rhythmic discharge of steroid-sensitive neurons
View evidence from paper
“the frequency of MUA volleys was reduced by gonadal steroids”