Source Paper
Neurokinin B and Dynorphin A in Kisspeptin Neurons of the Arcuate Nucleus Participate in Generation of Periodic Oscillation of Neural Activity Driving Pulsatile Gonadotropin-Releasing Hormone Secretion in the Goat
Yoshihiro Wakabayashi, Tomoaki Nakada, Ken Murata, Satoshi Ohkura, Kazutaka Mogi et al.
Journal of Neuroscience • 2010
Central Administration of Neurokinin B
Objective: To evaluate the effects of centrally administered neurokinin B on multiple-unit activity volleys and pulsatile luteinizing hormone secretion in the goat hypothalamus
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Equipment1
not specified • not specified • not specified • not mentioned
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Protocol Steps
Confirm coexpression of neuropeptides
Using double and triple labeling techniques to confirm that kisspeptin, neurokinin B, and dynorphin A are coexpressed in the same population of neurons in the arcuate nucleus
Note: This establishes the neuroanatomical basis for subsequent functional studies
View evidence from paper
“Using double and triple labeling, we confirmed that all three neuropeptides are coexpressed in the same population of neurons”
Record baseline multiple-unit activity volleys
Using electrophysiological techniques to record multiple-unit activity in the medial basal hypothalamus of ovariectomized animals to establish that bursts of MUA occur at regular intervals
Note: Recordings performed in ovariectomized animals; volleys are invariably associated with discrete pulses of luteinizing hormone
View evidence from paper
“Using electrophysiological techniques to record multiple-unit activity (MUA) in the medial basal hypothalamus, we found that bursts of MUA occurred at regular intervals in ovariectomized animals”
Assess effects of gonadal steroids on MUA frequency
Evaluate how gonadal steroids affect the frequency of MUA volleys to determine if volleys reflect rhythmic discharge of steroid-sensitive neurons
Note: Frequency of MUA volleys was reduced by gonadal steroids
View evidence from paper
“the frequency of MUA volleys was reduced by gonadal steroids, suggesting that the volleys reflect the rhythmic discharge of steroid-sensitive neurons”
Central administration of dynorphin A
Administer dynorphin A centrally and record effects on MUA volleys and pulsatile LH secretion
Note: Dynorphin A inhibits MUA volleys and pulsatile LH secretion
View evidence from paper
“central administration of Dyn-inhibit MUA volleys and pulsatile LH secretion”
Central administration of neurokinin B
Administer neurokinin B centrally and record effects on MUA volleys
Note: Neurokinin B induces MUA volleys
View evidence from paper
“NKB induced MUA volleys”