Cerebellar Control Infusion Study
Objective: Control experiment to verify that AP5 infusion effects on fear extinction are amygdala-specific by infusing AP5 into the interpositus nucleus of the cerebellum as a control site
Gather these items before starting the experiment. Check off items as you prepare.
Equipment2
Materials1
Not explicitly stated in provided text • Not applicable • Not provided • Not provided
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Protocol Steps
Prepare AP5 infusion
Prepare NMDA antagonist AP5 (D,L-2-amino-5-phosphonovaleric acid) for infusion
Note: This is a control infusion site experiment
View evidence from paper
“infusion of the NMDA antagonist D,L-2-amino-5-phosphonovaleric acid (AP5) into the amygdala”
Infuse AP5 into interpositus nucleus of cerebellum
Deliver AP5 infusion into the interpositus nucleus of the cerebellum as a control site to verify amygdala-specific effects
Note: This is the control site infusion; the interpositus nucleus is used as a control brain region
View evidence from paper
“Infusion of AP5 into the interpositus nucleus of the cerebellum, a control site, did not block extinction.”
Conduct fear-potentiated startle testing
Test fear extinction using the fear-potentiated startle paradigm following AP5 infusion into the cerebellum
Note: Measure whether extinction is blocked or proceeds normally
View evidence from paper
“the fear-potentiated startle paradigm was employed to begin to investigate neural mechanisms of extinction.”
Measure extinction outcome
Assess whether AP5 infusion into the interpositus nucleus blocks extinction of conditioned fear
Note: Control experiment to demonstrate blockade of extinction is amygdala-specific
View evidence from paper
“Infusion of AP5 into the interpositus nucleus of the cerebellum, a control site, did not block extinction.”