Cerebellar neurocan gene expression is a common pathogenic factor of trigeminal neuralgia and depression methods
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Open field test
The open field test is a widely recognized and classical experimental method used to assess the emotional behavior of animals. The experimental apparatus consisted of a rectangular open box measuring 100 cm × 100 cm × 40 cm, with white walls and a black floor. All animals were assessed on the 28 th day post-surgery. The experiment was performed in a quiet setting with dim lighting. Before the experiment, the rats were placed in the behavioral laboratory for one hour for adaptation. Each rat was gently placed in the central area of the open box and allowed to move freely for 5 min. Key observation indicators included total movement distance and movement speed. Following each trial, the box was promptly cleaned with low-concentration alcohol. Subsequent trials commenced only after the alcohol had fully evaporated.
Elevated plus maze test
The elevated plus maze (EPM) test is a well-established method for assessing emotional behavior in animals. The apparatus consisted of two enclosed arms (50 cm × 10 cm), two open arms (50 cm × 10 cm), and a central platform (10 cm × 10 cm). The entire apparatus was elevated 50 cm above the ground. Measurements were conducted on the 28 th day post-surgery for both the Sham and TN groups. Rats were placed in the center of the maze facing the open arms. Using animal behavior analysis software, the number of entries into the open arms and the duration of time spent in the open arms within a 5-minute period were recorded. Subsequently, the percentages of time spent in the open arms were calculated. The primary observation index was the percentage of time spent in the open arms, which served as an i...
Functional network properties analysis
This study employed the GRETNA toolbox and graph theory methods to systematically analyze the characteristics of brain network. Separate comparisons were conducted for the two patient groups, TN and depression, each against their own healthy controls. To investigate the variations in graph theory features across sparsity levels, this study computed the properties of brain regions exhibiting significant differences across a sparsity threshold range of 0.1-0.5 (step = 0.01) for the TN dataset and 0.1-0.48 (step = 0.01) for the depression dataset. Notably, the graph theory features of the TN group and the depression group demonstrated σ (σ > 1) throughout the entire connectivity density interval, with statistically significant differences. Futhermore, the analysis conducted using the NBS approach revealed no significant FC alterna...
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Evidence quotes (3)
The open field test is a widely recognized and classical experimental method used to assess the emotional behavior of animals. The experimental apparatus consisted of a rectangular open box measuring 100 cm × 100 cm × 40 cm, with white walls and a black floor. All animals were assessed on the 28 th day post-surgery. The experiment was performed in a quiet setting with dim lighting. Before the experiment, the rats were placed in the behavioral laboratory for one hour for adaptation. Each rat was gently placed in the central area of the open box and allowed to move freely for 5 min. Key observation indicators included total movement distance and movement speed. Following each trial, the box was promptly cleaned with low-concentration alcohol. Subsequent trials commenced only after the alcohol had fully evaporated.
The elevated plus maze (EPM) test is a well-established method for assessing emotional behavior in animals. The apparatus consisted of two enclosed arms (50 cm × 10 cm), two open arms (50 cm × 10 cm), and a central platform (10 cm × 10 cm). The entire apparatus was elevated 50 cm above the ground. Measurements were conducted on the 28 th day post-surgery for both the Sham and TN groups. Rats were placed in the center of the maze facing the open arms. Using animal behavior analysis software, the number of entries into the open arms and the duration of time spent in the open arms within a 5-minute period were recorded. Subsequently, the percentages of time spent in the open arms were calculated. The primary observation index was the percentage of time spent in the open arms, which served as an indicator for evaluating depressive-like behavior. The device was cleaned with low-concentration alcohol, and thoroughly dried prior to the next experiment.
This study employed the GRETNA toolbox and graph theory methods to systematically analyze the characteristics of brain network. Separate comparisons were conducted for the two patient groups, TN and depression, each against their own healthy controls. To investigate the variations in graph theory features across sparsity levels, this study computed the properties of brain regions exhibiting significant differences across a sparsity threshold range of 0.1-0.5 (step = 0.01) for the TN dataset and 0.1-0.48 (step = 0.01) for the depression dataset. Notably, the graph theory features of the TN group and the depression group demonstrated σ (σ > 1) throughout the entire connectivity density interval, with statistically significant differences. Futhermore, the analysis conducted using the NBS approach revealed no significant FC alternations in either TN patients or depression patients compared to healthy controls.
Machine-readable layer
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