Dopamine Agonist Disruption and Antipsychotic Drug Testing
Objective: Assessment of antipsychotic drug effects on dopamine agonist-induced disruptions of prepulse inhibition in zebrafish larvae
This is a Dopamine Agonist Disruption and Antipsychotic Drug Testing protocol using zebrafish as the model organism. The procedure involves 7 procedural steps, 1 equipment items, 2 materials. Extracted from a 2007 paper published in Journal of Neuroscience.
Model and subjects
zebrafish • Not specified • unknown • larval stage • Not specified
Study window
Estimated timing pending
Core workflow
Baseline startle response assessment • Prepulse inhibition testing • Dopamine agonist administration
Primary readouts
- Prepulse inhibition magnitude
- Acoustic startle response amplitude
- Sensory acuity in mutant lines
- Startle performance in mutant lines
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Baseline startle response assessment
Measure acoustic startle response in zebrafish larvae under control conditions
Note: Establishes baseline sensorimotor gating
View evidence from paper
“the acoustic startle response in zebrafish larvae is modulated by weak prepulses in a manner similar to mammalian PPI”
Prepulse inhibition testing
Apply weak prepulses to measure prepulse inhibition of the startle response
Note: Weak prepulses modulate startle response similar to mammalian PPI
View evidence from paper
“the acoustic startle response in zebrafish larvae is modulated by weak prepulses in a manner similar to mammalian PPI”
Dopamine agonist administration
Administer dopamine agonist to induce disruptions in prepulse inhibition
Note: Dopamine agonists disrupt PPI in zebrafish similar to mammals
View evidence from paper
“antipsychotic drugs can suppress disruptions in zebrafish PPI induced by dopamine agonists”
PPI measurement after dopamine agonist
Measure prepulse inhibition following dopamine agonist administration
Note: Quantifies degree of PPI disruption
View evidence from paper
“antipsychotic drugs can suppress disruptions in zebrafish PPI induced by dopamine agonists”
Antipsychotic drug administration
Administer antipsychotic drugs to test suppression of dopamine agonist-induced PPI disruptions
Note: Tests whether antipsychotics can reverse dopamine agonist effects
View evidence from paper
“antipsychotic drugs can suppress disruptions in zebrafish PPI induced by dopamine agonists”
PPI measurement after antipsychotic treatment
Measure prepulse inhibition following antipsychotic drug administration
Note: Determines efficacy of antipsychotic in restoring PPI
View evidence from paper
“antipsychotic drugs can suppress disruptions in zebrafish PPI induced by dopamine agonists”
Mutant line analysis
Analyze Ophelia mutant lines with reduced PPI for sensory acuity and startle performance
Note: Ophelia mutants have normal sensory acuity and startle but reduced PPI
View evidence from paper
“Ophelia mutants demonstrates that they have normal sensory acuity and startle performance, but reduced PPI”