Prepulse Inhibition of Startle Response
Objective: Measurement of sensorimotor gating by quantifying the modulation of acoustic startle response by weak prepulses in larval zebrafish
This is a Prepulse Inhibition of Startle Response protocol using zebrafish as the model organism. The procedure involves 6 procedural steps. Extracted from a 2007 paper published in Journal of Neuroscience.
Model and subjects
zebrafish • larval zebrafish • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Acoustic Startle Response Assessment • Prepulse Inhibition Testing • Pharmacological Testing with Dopamine Agonists
Primary readouts
- Acoustic startle response magnitude
- Prepulse inhibition percentage
- Modulation of startle response by weak prepulses
- Effects of dopamine agonists on PPI
Key equipment and reagents
Verified items
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Direct vendor links
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Protocol Steps
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Acoustic Startle Response Assessment
Measure the acoustic startle response in zebrafish larvae
Note: This is the baseline measurement before prepulse application
View evidence from paper
“the acoustic startle response in zebrafish larvae is modulated by weak prepulses”
Prepulse Inhibition Testing
Apply weak prepulses to modulate the startle response, measuring prepulse inhibition similar to mammalian PPI
Note: Weak prepulses precede the startle stimulus
View evidence from paper
“the acoustic startle response in zebrafish larvae is modulated by weak prepulses in a manner similar to mammalian PPI”
Pharmacological Testing with Dopamine Agonists
Induce disruptions in zebrafish PPI using dopamine agonists
Note: Used to test drug effects on sensorimotor gating
View evidence from paper
“antipsychotic drugs can suppress disruptions in zebrafish PPI induced by dopamine agonists”
Antipsychotic Drug Treatment
Apply antipsychotic drugs to suppress PPI disruptions induced by dopamine agonists
Note: Demonstrates pharmacological validity of the zebrafish PPI model
View evidence from paper
“antipsychotic drugs can suppress disruptions in zebrafish PPI induced by dopamine agonists”
Mutant Screening
Perform an unbiased screen to identify mutant lines with reduced PPI
Note: Isolated Ophelia mutants as part of genetic screen
View evidence from paper
“we performed a screen and isolated mutant lines with reduced PPI”
Ophelia Mutant Characterization
Analyze Ophelia mutants for sensory acuity, startle performance, and PPI levels
Note: Ophelia mutants show normal sensory acuity and startle performance but reduced PPI
View evidence from paper
“Ophelia mutants demonstrates that they have normal sensory acuity and startle performance, but reduced PPI”