Electrolytic Lesion of Rostral Midbrain Central Gray
Objective: Determine whether lesions of areas projected to by the central amygdaloid nucleus disrupt classical conditioning of autonomic and/or behavioral emotional responses, specifically testing effects of rostral midbrain central gray lesions on conditioned fear responses
This is a Electrolytic Lesion of Rostral Midbrain Central Gray protocol using Not explicitly stated as the model organism. The procedure involves 4 procedural steps, 5 equipment items, 1 materials. Extracted from a 1988 paper published in Journal of Neuroscience.
Model and subjects
Not explicitly stated • Not explicitly stated • Not explicitly stated • Not explicitly stated • Not explicitly stated • Not explicitly stated
Study window
Estimated timing pending
Core workflow
Surgical lesion creation • Recovery period • Classical conditioning
Primary readouts
- Conditioned arterial pressure response (autonomic measure)
- Conditioned freezing behavior (behavioral measure - arrest of somatomotor activity)
- Presence or absence of conditioned responses following lesions
Key equipment and reagents
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Protocol Steps
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Surgical lesion creation
Create electrolytic lesions of the rostral midbrain central gray region using electrolytic lesioning apparatus
Note: Lesions were made in the rostral central gray region specifically
View evidence from paper
“Electrolytic lesions of the rostral CG disrupted conditioned freezing but not conditioned changes in arterial pressure”
Recovery period
Allow animals to recover from surgical lesion procedure
Note: Recovery period before behavioral conditioning
View evidence from paper
“Two weeks later, the animals were classically conditioned by pairing an acoustic stimulus with footshock”
Classical conditioning
Pair acoustic stimulus with footshock to establish conditioned fear response
Note: Single pairing session described
View evidence from paper
“the animals were classically conditioned by pairing an acoustic stimulus with footshock”
Extinction trials and measurement
Present acoustic conditioned stimulus and measure conditioned changes in autonomic activity (arterial pressure increases) and behavioral responses (freezing) during extinction trials
Note: Both autonomic and behavioral measures were recorded simultaneously
View evidence from paper
“The next day, conditioned changes in autonomic activity (increases in arterial pressure) and emotional behavior ('freezing,' or the arrest of somatomotor activity) evoked by the acoustic conditioned stimulus (CS) were measured during extinction trials”