Source Paper
Neuroprotective Effects of Alpha-Lipoic Acid Against Behavioral Toxicity, Oxidative and Inflammatory Damage Caused by Titanium Dioxide Nanoparticles
Khedr MA, El-Kazaz SE, Rashed RR, Tohamy HG, Shukry M et al.
Biol Trace Elem Res • 2025
Elevated Plus Maze
Objective: To evaluate animals' anxiety levels using the Elevated Plus Maze test
This is a Elevated Plus Maze protocol using Rat as the model organism. The procedure involves 4 procedural steps, 1 equipment items, 1 materials. Extracted from a 2025 paper published in Biol Trace Elem Res.
Model and subjects
Rat • Sprague-Dawley • male • 3- to 4-month-old • 24
Study window
~5 minutes hands-on
Core workflow
Maze setup • Animal placement • Exploration period
Primary readouts
- Time elapsed to enter open or closed arms
- Time present in open and closed arms
- Open and closed arms entries (N)
- Time and entries in open and closed arms percent
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Maze setup
Set up maze with two open arms (50 × 10 cm) and two closed arms with high side walls (40 cm), connected by central platform (10 × 10 cm), elevated 50 cm above ground with 0.5 cm high edge around open arms
View evidence from paper
“The maze comprises two open arms (50 × 10 cm) and two closed arms of similar dimensions, with high side walls (40 cm)”
Animal placement
Position each rat in the central area facing an open arm
View evidence from paper
“Each rat was positioned in the central area, facing an open arm”
Exploration period
Allow rat to explore for 5 minutes before returning to cage
View evidence from paper
“allowed 5 min to explore before being returned to its cage”
Entry criteria definition
Define entry into central region as two paws on it, and arm entry as all four paws
View evidence from paper
“An entry into the central region was considered when two paws were on it, while all four paws were considered for arm entry”