Source Paper
Neuroprotective Effects of Alpha-Lipoic Acid Against Behavioral Toxicity, Oxidative and Inflammatory Damage Caused by Titanium Dioxide Nanoparticles
Khedr MA, El-Kazaz SE, Rashed RR, Tohamy HG, Shukry M et al.
Biol Trace Elem Res • 2025
Elevated Plus Maze
Objective: To evaluate animals' anxiety levels using the Elevated Plus Maze test
Gather these items before starting the experiment. Check off items as you prepare.
2 items available from ConductScience
Estimated: $3,780.00
As an Amazon Associate, we earn from qualifying purchases. Product links help support this free resource.
Protocol Steps
Maze setup
Set up maze with two open arms (50 × 10 cm) and two closed arms with high side walls (40 cm), connected by central platform (10 × 10 cm), elevated 50 cm above ground with 0.5 cm high edge around open arms
View evidence from paper
“The maze comprises two open arms (50 × 10 cm) and two closed arms of similar dimensions, with high side walls (40 cm)”
Animal placement
Position each rat in the central area facing an open arm
View evidence from paper
“Each rat was positioned in the central area, facing an open arm”
Exploration period
Allow rat to explore for 5 minutes before returning to cage
View evidence from paper
“allowed 5 min to explore before being returned to its cage”
Entry criteria definition
Define entry into central region as two paws on it, and arm entry as all four paws
View evidence from paper
“An entry into the central region was considered when two paws were on it, while all four paws were considered for arm entry”