Source Paper
Chronic Organic Magnesium Supplementation Enhances Tissue-Specific Bioavailability and Functional Capacity in Rats: A Focus on Brain, Muscle, and Vascular Health
Koc B, Hosgorler F, Kandis S, Acikgoz B, Kizildag S et al.
Biol Trace Elem Res • 2025
Elevated Plus Maze Test
Objective: To assess anxiety-like behavior in rats
Gather these items before starting the experiment. Check off items as you prepare.
1 item available from ConductScience
Estimated: $1,890.00
As an Amazon Associate, we earn from qualifying purchases. Product links help support this free resource.
Protocol Steps
Set up elevated plus maze
Position platform at 50 cm height with central square area (10×10 cm), two open arms (50 cm long, 10 cm wide, 1 cm high border) and two closed arms (50 cm long, 10 cm wide, 50 cm high walls)
View evidence from paper
“The height of the platform used for the elevated plus maze test was 50 cm”
Place subject in maze
Place rat in the middle of the central platform facing one of the open arms
View evidence from paper
“The subject was placed in the middle of the central platform, facing one of the open arms”
Monitor behavior
Record rat behavior with camera for 300 seconds
View evidence from paper
“monitored with a camera for 300 s”
Clean maze between trials
Clean the maze with 70% ethanol between each trial
View evidence from paper
“The maze was cleaned with 70% ethanol between each trial”
Record behavioral parameters
Record total number of entries to arms and total time spent in arms using video tracking software
View evidence from paper
“The total number of entries to arms and total time spent in arms were recorded via Noldus Ethovision XT software”