Source Paper
Experience-Specific Functional Modification of the Dentate Gyrus through Adult Neurogenesis: A Critical Period during an Immature Stage
Ayumu Tashiro, Hiroshi Makino, Fred H. Gage
Journal of Neuroscience • 2007
Enriched Environment Exposure
Objective: To determine whether experience-dependent anatomical modification through adult neurogenesis alters functional properties of the dentate gyrus, and to identify critical periods during which new neurons are sensitive to environmental experience
This is a Enriched Environment Exposure protocol using Not explicitly stated in provided text as the model organism. The procedure involves 3 procedural steps, 1 equipment items. Extracted from a 2007 paper published in Journal of Neuroscience.
Model and subjects
Not explicitly stated in provided text • Not explicitly stated in provided text • unknown • Not explicitly stated in provided text • Not explicitly stated in provided text
Study window
~3 week study window
Core workflow
Enriched environment exposure • Reexposure to same environment • Immediate early gene expression analysis
Primary readouts
- Total number of new neurons in dentate gyrus
- Number of new neurons responding to reexposure to same environment
- Density of activated new neurons
- c-fos expression patterns
Key equipment and reagents
Verified items
0
Direct vendor links
0
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Enriched environment exposure
Animals exposed to an enriched environment during specified developmental period
Note: Experience-specific modifications are affected exclusively by previous exposure around the second week after neuronal birth but not later than 3 weeks
View evidence from paper
“these experience-specific modifications are affected exclusively by previous exposure around the second week after neuronal birth but not later than 3 weeks”
Reexposure to same environment
Animals reexposed to the same enriched environment previously experienced
Note: Increase in density of activated new neurons occurred specifically in response to exposure to the same environment but not to a different experience
View evidence from paper
“The increase in the density of activated new neurons occurred specifically in response to exposure to the same environment but not to a different experience”
Immediate early gene expression analysis
Assessment of c-fos and Zif268 expression as indicators of recently activated neurons
Note: c-fos and Zif268 used as markers of recently activated neurons to measure functional response
View evidence from paper
“using the expression of immediate early gene products, c-fos and Zif268, as indicators of recently activated neurons”