Source Paper
Erythropoietin Regulates the<i>In Vitro</i>and<i>In Vivo</i>Production of Neuronal Progenitors by Mammalian Forebrain Neural Stem Cells
Tetsuro Shingo, S. Todd Sorokan, Takuya Shimazaki, Samuel Weiss
Journal of Neuroscience • 2001
EPO Infusion into Lateral Ventricles
Objective: To measure the effects of EPO infusion into adult lateral ventricles on neural stem cells in the subventricular zone and neurogenesis in the olfactory bulb
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Equipment1
Not specified in provided text • Not mentioned • Not mentioned • Not mentioned
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Protocol Steps
Prepare EPO or anti-EPO antibody solution
Prepare erythropoietin or anti-EPO antibody solution for infusion into lateral ventricles
Note: Two treatment conditions: EPO infusion and anti-EPO antibody infusion
View evidence from paper
“EPO infusion into the adult lateral ventricles resulted in a decrease in the numbers of NSCs in the subventricular zone, an increase in newly generated cells migrating to the olfactory bulb”
Infuse EPO into lateral ventricles
Administer EPO infusion into adult lateral ventricles to promote neurogenesis and neural stem cell differentiation
Note: Results in decreased NSCs in subventricular zone and increased newly generated cells migrating to olfactory bulb
View evidence from paper
“EPO infusion into the adult lateral ventricles resulted in a decrease in the numbers of NSCs in the subventricular zone, an increase in newly generated cells migrating to the olfactory bulb, and an increase in new olfactory bulb interneurons”
Infuse anti-EPO antibodies into lateral ventricles (control condition)
Administer anti-EPO antibody infusion into adult lateral ventricles as control to block endogenous EPO effects
Note: Produces opposite effects to EPO infusion
View evidence from paper
“Infusion of anti-EPO antibodies had the opposite effect: an increase in the number of NSCs in the subventricular zone and a decrease in the number of newly generated cells migrating to the bulb”
Assess neural stem cell numbers in subventricular zone
Quantify the number of neural stem cells remaining in the subventricular zone following infusion
Note: EPO infusion decreases NSC numbers; anti-EPO antibody infusion increases NSC numbers
View evidence from paper
“EPO infusion into the adult lateral ventricles resulted in a decrease in the numbers of NSCs in the subventricular zone”
Measure newly generated cells migrating to olfactory bulb
Quantify the number of newly generated cells that have migrated from the subventricular zone to the olfactory bulb
Note: EPO infusion increases migration; anti-EPO antibody infusion decreases migration
View evidence from paper
“an increase in newly generated cells migrating to the olfactory bulb, and an increase in new olfactory bulb interneurons”
Count new olfactory bulb interneurons
Quantify the number of newly generated interneurons in the olfactory bulb
Note: EPO infusion increases new interneuron production
View evidence from paper
“an increase in new olfactory bulb interneurons”