Source Paper
Memory Reconsolidation and Extinction Have Distinct Temporal and Biochemical Signatures
Akinobu Suzuki, Sheena A. Josselyn, Paul W. Frankland, Shoichi Masushige, Alcino J. Silva et al.
Journal of Neuroscience • 2004
Fear Conditioning
Objective: To induce and measure memory formation and reconsolidation in response to fear-associated stimuli, and to examine the temporal dynamics and biochemical signatures of memory reconsolidation versus extinction
This is a Fear Conditioning protocol using Not explicitly stated in provided text as the model organism. The procedure involves 3 procedural steps. Extracted from a 2004 paper published in Journal of Neuroscience.
Model and subjects
Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text
Study window
Estimated timing pending
Core workflow
Fear Conditioning Paradigm • Memory Retrieval and Reconsolidation Assessment • Pharmacological Manipulation
Primary readouts
- Memory reconsolidation strength
- Memory extinction
- Temporal dynamics of memory processing after retrieval
- Biochemical signatures of reconsolidation versus extinction
Key equipment and reagents
Verified items
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Direct vendor links
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Protocol Steps
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Fear Conditioning Paradigm
Subjects undergo fear conditioning to establish memory formation in response to fear-associated stimuli
Note: The study examines how memory strength and age affect reconsolidation processes
View evidence from paper
“Memory retrieval is not a passive phenomenon. Instead, it triggers a number of processes that either reinforce or alter stored information.”
Memory Retrieval and Reconsolidation Assessment
Memory retrieval is triggered to activate reconsolidation cascade that requires protein synthesis
Note: Temporal dynamics of reconsolidation depend on strength and age of memory
View evidence from paper
“Retrieval is thought to activate a second memory consolidation cascade (reconsolidation) that requires protein synthesis.”
Pharmacological Manipulation
Antagonism of cannabinoid receptor 1 or L-type voltage-gated calcium channels is applied to test differential effects on extinction versus reconsolidation
Note: These manipulations block extinction but not reconsolidation, demonstrating distinct biochemical signatures
View evidence from paper
“Pharmacological antagonism of either cannabinoid receptor 1 or L-type voltage-gated calcium channels blocks extinction but not reconsolidation.”