Femoral Artery Occlusion
Objective: To investigate monocyte accumulation and its association with cytokine production (bFGF and TNF-alpha) and vessel proliferation during collateral vessel growth and angiogenesis following femoral artery occlusion in rabbits
This is a Femoral Artery Occlusion protocol using rabbit as the model organism. The procedure involves 6 procedural steps, 1 materials. Extracted from a 1998 paper published in Journal of Clinical Investigation.
Model and subjects
rabbit • not specified • unknown • not specified • not specified
Study window
~1 week study window
Core workflow
Femoral Artery Occlusion Surgery • LPS Treatment Administration • Monocyte Accumulation Assessment at Day 3
Primary readouts
- Monocyte accumulation in collateral arteries
- Macrophage accumulation in lower limb
- bFGF and TNF-alpha staining intensity
- Vessel proliferation rate
Key equipment and reagents
Verified items
0
Direct vendor links
0
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Femoral Artery Occlusion Surgery
Surgical occlusion of the femoral artery in rabbits to induce collateral vessel growth and angiogenesis
Note: This is the primary intervention to initiate the experimental model
View evidence from paper
“after femoral artery occlusion”
LPS Treatment Administration
Administration of LPS to increase monocyte recruitment in treatment group
Note: Applied to treatment group only; control group remained untreated
View evidence from paper
“LPS treatment significantly increased capillary density and peripheral conductance as compared with untreated animals”
Monocyte Accumulation Assessment at Day 3
Evaluation of monocyte accumulation in collateral arteries around day 3 post-occlusion when maximal proliferation was observed
Note: Monocytes stained for bFGF and TNF-alpha at this timepoint
View evidence from paper
“Monocytes accumulated around day 3 in collateral arteries when maximal proliferation was observed, and stained strongly for bFGF and TNF-alpha”
Macrophage Accumulation Assessment at Day 7
Evaluation of macrophage accumulation in lower limb where angiogenesis is predominant, around day 7 post-occlusion
Note: Macrophage accumulation closely associated with maximal proliferation in angiogenic regions
View evidence from paper
“In the lower limb where angiogenesis was shown to be predominant, macrophage accumulation was also closely associated with maximal proliferation (around day 7)”
Capillary Density Measurement
Measurement of capillary density at 7 days post-occlusion in both treated and untreated animals
Note: LPS-treated animals showed significantly increased capillary density compared to controls
View evidence from paper
“LPS treatment significantly increased capillary density (424+/-26.1 n/mm2 vs. 312+/-20.7 n/mm2; P < 0.05)”
Peripheral Conductance Measurement
Measurement of peripheral conductance at 7 days post-occlusion in both treated and untreated animals
Note: LPS-treated animals showed significantly increased peripheral conductance compared to controls
View evidence from paper
“LPS treatment significantly increased peripheral conductance (109+/-33.8 ml/min/100 mmHg vs. 45+/-6.8 ml/min/100 mmHg; P < 0.05)”