Femoral Artery Occlusion
Objective: To investigate monocyte accumulation and its association with cytokine production (bFGF and TNF-alpha) and vessel proliferation during collateral vessel growth and angiogenesis following femoral artery occlusion in rabbits
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Materials1
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Protocol Steps
Femoral Artery Occlusion Surgery
Surgical occlusion of the femoral artery in rabbits to induce collateral vessel growth and angiogenesis
Note: This is the primary intervention to initiate the experimental model
View evidence from paper
“after femoral artery occlusion”
LPS Treatment Administration
Administration of LPS to increase monocyte recruitment in treatment group
Note: Applied to treatment group only; control group remained untreated
View evidence from paper
“LPS treatment significantly increased capillary density and peripheral conductance as compared with untreated animals”
Monocyte Accumulation Assessment at Day 3
Evaluation of monocyte accumulation in collateral arteries around day 3 post-occlusion when maximal proliferation was observed
Note: Monocytes stained for bFGF and TNF-alpha at this timepoint
View evidence from paper
“Monocytes accumulated around day 3 in collateral arteries when maximal proliferation was observed, and stained strongly for bFGF and TNF-alpha”
Macrophage Accumulation Assessment at Day 7
Evaluation of macrophage accumulation in lower limb where angiogenesis is predominant, around day 7 post-occlusion
Note: Macrophage accumulation closely associated with maximal proliferation in angiogenic regions
View evidence from paper
“In the lower limb where angiogenesis was shown to be predominant, macrophage accumulation was also closely associated with maximal proliferation (around day 7)”
Capillary Density Measurement
Measurement of capillary density at 7 days post-occlusion in both treated and untreated animals
Note: LPS-treated animals showed significantly increased capillary density compared to controls
View evidence from paper
“LPS treatment significantly increased capillary density (424+/-26.1 n/mm2 vs. 312+/-20.7 n/mm2; P < 0.05)”
Peripheral Conductance Measurement
Measurement of peripheral conductance at 7 days post-occlusion in both treated and untreated animals
Note: LPS-treated animals showed significantly increased peripheral conductance compared to controls
View evidence from paper
“LPS treatment significantly increased peripheral conductance (109+/-33.8 ml/min/100 mmHg vs. 45+/-6.8 ml/min/100 mmHg; P < 0.05)”