Vascular Conductance Assessment
Objective: Measurement of collateral and peripheral vascular conductance in the rabbit hindlimb after femoral artery occlusion, and investigation of monocyte accumulation's association with cytokine production and vessel proliferation
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
not specified • not specified • not specified • not mentioned
Materials1
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Protocol Steps
Femoral artery occlusion
Perform femoral artery occlusion in rabbit hindlimb
Note: Baseline procedure for vascular conductance assessment
View evidence from paper
“after femoral artery occlusion”
LPS treatment (experimental group)
Administer LPS treatment to increase monocyte recruitment in experimental animals
Note: Applied to experimental group only; control group remains untreated
View evidence from paper
“we studied the effects of an increase in monocyte recruitment by LPS”
Monocyte accumulation assessment
Assess monocyte accumulation around day 3 in collateral arteries and around day 7 in lower limb
Note: Monocytes stained for bFGF and TNF-alpha
View evidence from paper
“Monocytes accumulated around day 3 in collateral arteries when maximal proliferation was observed, and stained strongly for bFGF and TNF-alpha”
Capillary density measurement
Measure capillary density in hindlimb tissue
Note: Measured in n/mm2 units
View evidence from paper
“LPS treatment significantly increased capillary density (424+/-26.1 n/mm2 vs. 312+/-20.7 n/mm2; P < 0.05)”
Peripheral conductance measurement
Measure peripheral vascular conductance in rabbit hindlimb
Note: Measured in ml/min/100 mmHg units
View evidence from paper
“peripheral conductance (109+/-33.8 ml/min/100 mmHg vs. 45+/-6.8 ml/min/100 mmHg; P < 0.05)”
Collateral conductance measurement
Measure collateral vascular conductance in rabbit hindlimb
Note: Assessment of collateral artery growth
View evidence from paper
“collateral and peripheral conductance after 7 d of occlusion”