Vascular Conductance Assessment
Objective: Measurement of collateral and peripheral vascular conductance in the rabbit hindlimb after femoral artery occlusion, and investigation of monocyte accumulation's association with cytokine production and vessel proliferation
This is a Vascular Conductance Assessment protocol using rabbit as the model organism. The procedure involves 6 procedural steps, 1 equipment items, 1 materials. Extracted from a 1998 paper published in Journal of Clinical Investigation.
Model and subjects
rabbit • not specified • unknown • not specified • not specified
Study window
~1 week study window
Core workflow
Femoral artery occlusion • LPS treatment (experimental group) • Monocyte accumulation assessment
Primary readouts
- Capillary density (n/mm2)
- Peripheral vascular conductance (ml/min/100 mmHg)
- Collateral vascular conductance
- Monocyte accumulation patterns
Key equipment and reagents
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Protocol Steps
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Femoral artery occlusion
Perform femoral artery occlusion in rabbit hindlimb
Note: Baseline procedure for vascular conductance assessment
View evidence from paper
“after femoral artery occlusion”
LPS treatment (experimental group)
Administer LPS treatment to increase monocyte recruitment in experimental animals
Note: Applied to experimental group only; control group remains untreated
View evidence from paper
“we studied the effects of an increase in monocyte recruitment by LPS”
Monocyte accumulation assessment
Assess monocyte accumulation around day 3 in collateral arteries and around day 7 in lower limb
Note: Monocytes stained for bFGF and TNF-alpha
View evidence from paper
“Monocytes accumulated around day 3 in collateral arteries when maximal proliferation was observed, and stained strongly for bFGF and TNF-alpha”
Capillary density measurement
Measure capillary density in hindlimb tissue
Note: Measured in n/mm2 units
View evidence from paper
“LPS treatment significantly increased capillary density (424+/-26.1 n/mm2 vs. 312+/-20.7 n/mm2; P < 0.05)”
Peripheral conductance measurement
Measure peripheral vascular conductance in rabbit hindlimb
Note: Measured in ml/min/100 mmHg units
View evidence from paper
“peripheral conductance (109+/-33.8 ml/min/100 mmHg vs. 45+/-6.8 ml/min/100 mmHg; P < 0.05)”
Collateral conductance measurement
Measure collateral vascular conductance in rabbit hindlimb
Note: Assessment of collateral artery growth
View evidence from paper
“collateral and peripheral conductance after 7 d of occlusion”