Focal X-ray Irradiation of Hippocampus
Objective: To ablate hippocampal neurogenesis using focal X-ray irradiation and assess effects on contextual fear conditioning, spatial learning, and synaptic plasticity in the dentate gyrus
This is a Focal X-ray Irradiation of Hippocampus protocol using Not explicitly stated in provided text as the model organism. The procedure involves 5 procedural steps, 3 equipment items. Extracted from a 2006 paper published in Proceedings of the National Academy of Sciences.
Model and subjects
Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text
Study window
Estimated timing pending
Core workflow
Focal X-ray irradiation of hippocampus • Contextual fear conditioning test • Morris water maze spatial learning task
Primary readouts
- Contextual fear conditioning performance
- Cued fear conditioning performance
- Morris water maze spatial learning
- Y maze spatial learning
Key equipment and reagents
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Protocol Steps
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Focal X-ray irradiation of hippocampus
Deliver focal X-ray irradiation to the hippocampus to ablate neurogenesis
Note: This is one of two independent methods used to ablate hippocampal neurogenesis
View evidence from paper
“focal X irradiation of the hippocampus or genetic ablation of glial fibrillary acidic protein-positive neural progenitor cells”
Contextual fear conditioning test
Assess contextual fear conditioning in animals following hippocampal neurogenesis ablation
Note: Contextual fear conditioning was impaired by ablation procedures, but cued conditioning was not affected
View evidence from paper
“focal X irradiation of the hippocampus or genetic ablation of glial fibrillary acidic protein-positive neural progenitor cells impaired contextual fear conditioning but not cued conditioning”
Morris water maze spatial learning task
Assess hippocampal-dependent spatial learning using Morris water maze
Note: Morris water maze performance was unaffected by neurogenesis ablation
View evidence from paper
“Hippocampal-dependent spatial learning tasks such as the Morris water maze and Y maze were unaffected”
Y maze spatial learning task
Assess hippocampal-dependent spatial learning using Y maze
Note: Y maze performance was unaffected by neurogenesis ablation
View evidence from paper
“Hippocampal-dependent spatial learning tasks such as the Morris water maze and Y maze were unaffected”
Electrophysiological assessment of long-term potentiation in dentate gyrus
Measure long-term potentiation elicited in the dentate gyrus in the absence of GABA blockers to assess synaptic plasticity
Note: Long-term potentiation in dentate gyrus requires presence of new neurons and is eliminated by ablation procedures
View evidence from paper
“long-term potentiation elicited in the dentate gyrus in the absence of GABA blockers requires the presence of new neurons, as it is eliminated by each of our ablation procedures”