Focal X-ray Irradiation of Hippocampus
Objective: To ablate hippocampal neurogenesis using focal X-ray irradiation and assess effects on contextual fear conditioning, spatial learning, and synaptic plasticity in the dentate gyrus
Gather these items before starting the experiment. Check off items as you prepare.
Equipment3
Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text
Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text
Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text
2 items available from ConductScience
Estimated: $1,295.00
As an Amazon Associate, we earn from qualifying purchases. Product links help support this free resource.
Protocol Steps
Focal X-ray irradiation of hippocampus
Deliver focal X-ray irradiation to the hippocampus to ablate neurogenesis
Note: This is one of two independent methods used to ablate hippocampal neurogenesis
View evidence from paper
“focal X irradiation of the hippocampus or genetic ablation of glial fibrillary acidic protein-positive neural progenitor cells”
Contextual fear conditioning test
Assess contextual fear conditioning in animals following hippocampal neurogenesis ablation
Note: Contextual fear conditioning was impaired by ablation procedures, but cued conditioning was not affected
View evidence from paper
“focal X irradiation of the hippocampus or genetic ablation of glial fibrillary acidic protein-positive neural progenitor cells impaired contextual fear conditioning but not cued conditioning”
Morris water maze spatial learning task
Assess hippocampal-dependent spatial learning using Morris water maze
Note: Morris water maze performance was unaffected by neurogenesis ablation
View evidence from paper
“Hippocampal-dependent spatial learning tasks such as the Morris water maze and Y maze were unaffected”
Y maze spatial learning task
Assess hippocampal-dependent spatial learning using Y maze
Note: Y maze performance was unaffected by neurogenesis ablation
View evidence from paper
“Hippocampal-dependent spatial learning tasks such as the Morris water maze and Y maze were unaffected”
Electrophysiological assessment of long-term potentiation in dentate gyrus
Measure long-term potentiation elicited in the dentate gyrus in the absence of GABA blockers to assess synaptic plasticity
Note: Long-term potentiation in dentate gyrus requires presence of new neurons and is eliminated by ablation procedures
View evidence from paper
“long-term potentiation elicited in the dentate gyrus in the absence of GABA blockers requires the presence of new neurons, as it is eliminated by each of our ablation procedures”