Source Paper
Limbic and Motor Circuitry Underlying Footshock-Induced Reinstatement of Cocaine-Seeking Behavior
Krista McFarland, Susan B. Davidge, Christopher C. Lapish, Peter W. Kalivas
Journal of Neuroscience • 2004
Materials & Equipment Checklist
5 items1 from ConductScience
Gather these items before starting the experiment. Check off items as you prepare.
Equipment4
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Materials1
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Estimated: $4,900.00
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Protocol Steps
1
Cocaine self-administration training
Animals are trained to self-administer cocaine by lever pressing in operant conditioning chambers
Not explicitly statedNot explicitly stated
Drosophila Olfactory Operant Conditioning
Matches: Operant conditioning chambers for cocaine self-administration
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Note: Establishes baseline lever pressing behavior associated with cocaine delivery
View evidence from paper
“lever pressing previously associated with cocaine delivery”
2
Extinction training
Lever pressing is no longer reinforced with cocaine to extinguish the drug-seeking response
Not explicitly statedNot explicitly stated
Drosophila Olfactory Operant Conditioning
Matches: Operant conditioning chambers for cocaine self-administration
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Note: Establishes extinction baseline before reinstatement testing
View evidence from paper
“Not explicitly stated in provided text”
3
Transient inhibition of central extended amygdala
Transient inhibition of the central extended amygdala (CEA), including the central nucleus of the amygdala (CN), ventral bed nucleus of the stria terminalis (BNSTv), and nucleus accumbens shell (NAshell)
Not explicitly statedNot explicitly stated
Note: This inhibition blocks the ability of footshock stress to reinstate lever pressing
View evidence from paper
“Transient inhibition of the central extended amygdala [CEA; including the central nucleus of the amygdala (CN), ventral bed nucleus of the stria terminalis (BNSTv), and nucleus accumbens shell (NAshell)] blocked the ability of footshock stress to reinstate lever pressing”
4
Transient inhibition of ventral tegmental area
Transient inhibition of the ventral tegmental area (VTA)
Not explicitly statedNot explicitly stated
Note: VTA inhibition blocks footshock-induced reinstatement of lever pressing
View evidence from paper
“ventral tegmental area (VTA) blocked the ability of footshock stress to reinstate lever pressing”
5
Transient inhibition of motor circuitry
Transient inhibition of motor circuitry including the dorsal prefrontal cortex (PFCd), nucleus accumbens core (NAcore), and ventral pallidum (VP)
Not explicitly statedNot explicitly stated
Note: Motor circuitry inhibition blocks footshock-induced reinstatement
View evidence from paper
“motor circuitry [including the dorsal prefrontal cortex (PFCd), nucleus accumbens core (NAcore), and ventral pallidum (VP)] blocked the ability of footshock stress to reinstate lever pressing”
6
Fluphenazine infusion into prefrontal cortex
Infuse D1/D2 dopamine receptor antagonist fluphenazine into the dorsal prefrontal cortex (PFCd)
Not explicitly statedNot explicitly stated
Note: Fluphenazine blocks footshock-induced reinstatement when infused into the PFCd
View evidence from paper
“The D1/D2 dopamine receptor antagonist fluphenazine blocked footshock-induced reinstatement when infused into the PFCd”
7
Footshock stress application
Apply footshock stress to animals to induce reinstatement of cocaine-seeking behavior
Not explicitly statedNot explicitly stated
Note: Footshock activates limbic circuitry in the CEA
View evidence from paper
“footshock stress activates limbic circuitry of the CEA”
8
Measure lever pressing reinstatement
Measure the number of lever presses following footshock stress to quantify reinstatement of cocaine-seeking behavior
Not explicitly statedNot explicitly stated
Note: Primary outcome measure for reinstatement
View evidence from paper
“blocked the ability of footshock stress to reinstate lever pressing”
9
Measure dopamine levels in prefrontal cortex
Measure footshock-induced increase in dopamine within the prefrontal cortex (PFC)
Not explicitly statedNot explicitly stated
Note: Inhibition of NAshell blocks this dopamine increase and concomitantly blocks reinstatement
View evidence from paper
“inhibition of the NAshell blocked a footshock-induced increase in dopamine within the PFC and concomitantly blocked reinstatement responding”
10
Measure glutamate release in nucleus accumbens core
Measure stress-induced glutamate release within the nucleus accumbens core (NAcore)
Not explicitly statedNot explicitly stated
Note: Inactivation of PFCd blocks stress-induced glutamate release in NAcore while inhibiting reinstatement
View evidence from paper
“inactivation of the PFCd was shown to block stress-induced glutamate release within the NAcore while concurrently inhibiting reinstatement responding”
Subjects / Specimens
- Species
- Not explicitly stated in provided text
- Strain
- Not explicitly stated in provided text
- Age
- Not explicitly stated in provided text
- Sex
- unknown
- Weight
- Not explicitly stated in provided text
Not explicitly stated in provided text