Source Paper
Functional assessments in the rodent stroke model
Krystal L Schaar, Miranda M Brenneman, Sean I Savitz
Experimental & Translational Stroke Medicine • 2010
View Abstract
Abstract Stroke is a common cause of permanent disability accompanied by devastating impairments for which there is a pressing need for effective treatment. Motor, sensory and cognitive deficits are common following stroke, yet treatment is limited. Along with histological measures, functional outcome in animal models has provided valuable insight to the biological basis and potential rehabilitation efforts of experimental stroke. Developing and using tests that have the ability to identify behavioral deficits is essential to expanding the development of translational therapies. The present aim of this paper is to review many of the current behavioral tests that assess functional outcome after stoke in rodent models. While there is no perfect test, there are many assessments that are sensitive to detecting the array of impairments, from global to modality specific, after stroke.
Forelimb Placing Test
Objective: Assessment of forelimb function and placing deficits in rodent stroke models
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
Not specified • Not specified • Not specified • Not specified
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Protocol Steps
Animal Preparation
Prepare rodent for testing following stroke induction via unilateral MCAO
Note: Testing should be performed after stroke has been induced to assess contralateral neurological deficits
View evidence from paper
“Unilateral middle cerebral artery occlusion (MCAO) in both humans and rodents induces contralateral neurological deficits”
Conduct Forelimb Placing Test
Perform the forelimb placing test to evaluate forelimb function and placing deficits
Note: No pre-training is required for this test. The test involves 10 trials per session.
View evidence from paper
“Forelimb Placing None 10 trials 5 minutes”
Record Observations
Document forelimb placing responses and any deficits observed during the test
Note: Assess for asymmetries in forelimb placing function between contralateral and ipsilateral sides
View evidence from paper
“Unilateral brain damage results in deficits of symmetry, therefore it is useful to rely on tests that have the ability to detect asymmetries”