Source Paper
Brain-Derived Neurotrophic Factor and Tyrosine Kinase Receptor B Involvement in Amygdala-Dependent Fear Conditioning
Lisa M. Rattiner, Michael Davis, Christopher T. French, Kerry J. Ressler
Journal of Neuroscience • 2004
Intra-amygdala Infusion of K252a
Objective: To assess the role of Trk receptor signaling in amygdala-dependent fear conditioning acquisition by disrupting neurotrophin signaling with intra-amygdala infusion of K252a
Gather these items before starting the experiment. Check off items as you prepare.
Equipment2
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Materials1
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Protocol Steps
Intra-amygdala infusion of K252a
Administer intra-amygdala infusion of the Trk receptor antagonist K252a to disrupt neurotrophin signaling
Note: This infusion is performed prior to or during fear conditioning to assess its effect on acquisition
View evidence from paper
“disruption of neurotrophin signaling with intra-amygdala infusion of the Trk receptor antagonist K252a disrupted acquisition of fear conditioning”
Pavlovian fear conditioning
Conduct Pavlovian fear conditioning as the learning model to examine the role of BDNF in the amygdala
Note: Fear conditioning occurs after K252a infusion to measure acquisition disruption
View evidence from paper
“the use of Pavlovian fear conditioning as a learning model allows us to examine the concise role of BDNF in the amygdala after a single learning session”
Measure fear acquisition
Assess whether K252a infusion disrupted the acquisition of conditioned fear responses
Note: Outcome measure is whether fear conditioning acquisition is disrupted by K252a treatment
View evidence from paper
“disruption of neurotrophin signaling with intra-amygdala infusion of the Trk receptor antagonist K252a disrupted acquisition of fear conditioning”