Source Paper
Brain-Derived Neurotrophic Factor and Tyrosine Kinase Receptor B Involvement in Amygdala-Dependent Fear Conditioning
Lisa M. Rattiner, Michael Davis, Christopher T. French, Kerry J. Ressler
Journal of Neuroscience • 2004
Intra-amygdala Infusion of K252a
Objective: To assess the role of Trk receptor signaling in amygdala-dependent fear conditioning acquisition by disrupting neurotrophin signaling with intra-amygdala infusion of K252a
This is a Intra-amygdala Infusion of K252a protocol using Not explicitly stated in provided text as the model organism. The procedure involves 3 procedural steps, 2 equipment items, 1 materials. Extracted from a 2004 paper published in Journal of Neuroscience.
Model and subjects
Not explicitly stated in provided text • Not explicitly stated in provided text • unknown • Not explicitly stated in provided text • Not explicitly stated in provided text
Study window
Estimated timing pending
Core workflow
Intra-amygdala infusion of K252a • Pavlovian fear conditioning • Measure fear acquisition
Primary readouts
- Acquisition of fear conditioning (disrupted vs. normal)
- Conditioned fear response magnitude
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
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- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Intra-amygdala infusion of K252a
Administer intra-amygdala infusion of the Trk receptor antagonist K252a to disrupt neurotrophin signaling
Note: This infusion is performed prior to or during fear conditioning to assess its effect on acquisition
View evidence from paper
“disruption of neurotrophin signaling with intra-amygdala infusion of the Trk receptor antagonist K252a disrupted acquisition of fear conditioning”
Pavlovian fear conditioning
Conduct Pavlovian fear conditioning as the learning model to examine the role of BDNF in the amygdala
Note: Fear conditioning occurs after K252a infusion to measure acquisition disruption
View evidence from paper
“the use of Pavlovian fear conditioning as a learning model allows us to examine the concise role of BDNF in the amygdala after a single learning session”
Measure fear acquisition
Assess whether K252a infusion disrupted the acquisition of conditioned fear responses
Note: Outcome measure is whether fear conditioning acquisition is disrupted by K252a treatment
View evidence from paper
“disruption of neurotrophin signaling with intra-amygdala infusion of the Trk receptor antagonist K252a disrupted acquisition of fear conditioning”