Inverted U-Shaped Dose-Response Curve of the Anxiolytic Effect of Cannabidiol during Public Speaking in Real Life methods
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Materials and Methods
Sixty healthy men and women aged 18-35 years, with no history of past or current psychiatric illness, alcohol or other drug dependence were recruited through advertisement in the campus of the University of São Paulo in Ribeirão Preto. Participants with major medical conditions or who were taking medications with the potential to interfere with the study's results were not included. The volunteers were interviewed in order to assess eligibility and to measure their propensity to anxiety through the trait version of the Spielberger State-Trait Anxiety Inventory (STAI- ). Eligible participants who agreed to participate were instructed to abstain from drugs in the week prior to the tests and from alcohol and caffeine in the 24 h before the test. All volunteers gave written informed consent to participate after being fully informed of the research procedures, which c...
Materials and Methods
The volunteers were randomly allocated to five groups with 12 subjects each to receive different doses of CBD (100, 300, and 900 mg), clonazepam (1 mg) or placebo in a double-blind, randomized design. The groups were matched according to gender, age, body mass index (BMI), and STAI-trait score. One volunteer from the CBD-300 group could not attend the experimental session due to personal issues and was withdrawn from the study.
Procedure
Volunteers began the experimental session after a minimum of 6 h of sleep and 2 h after eating a standard breakfast (200 ml chocolate milk and 100 g of bread with margarine).
Procedure
The 12 volunteers sat in chairs arranged in a semicircle, following a predetermined order by the treatment number assigned to each one of them and performed the baseline measurements, followed by the intake of the five capsules within a 5-min interval for each volunteer. This phase was completed over a period of 1 h. The volunteers then watched a film about birds of Amazon forest lasting about 20 min. After that, the measurements were repeated with an interval of 5 min for each subject (pretest measure). 2 h and 20 min after the first volunteer had received the treatment, they all watched a video lasting about 10 min with recorded instructions for the speech test. 2 h and 30 min after the first volunteer received the treatment, they started performing the speech test, which lasted 5 min. Subsequently, the other volunteers were submitted to the speech test in the same order in which th...
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Sixty healthy men and women aged 18-35 years, with no history of past or current psychiatric illness, alcohol or other drug dependence were recruited through advertisement in the campus of the University of São Paulo in Ribeirão Preto. Participants with major medical conditions or who were taking medications with the potential to interfere with the study's results were not included. The volunteers were interviewed in order to assess eligibility and to measure their propensity to anxiety through the trait version of the Spielberger State-Trait Anxiety Inventory (STAI- ). Eligible participants who agreed to participate were instructed to abstain from drugs in the week prior to the tests and from alcohol and caffeine in the 24 h before the test. All volunteers gave written informed consent to participate after being fully informed of the research procedures, which conformed to the current terms of the Declaration of Helsinki and were approved by the Ethics Committee of the Ribeirão Preto Medical School University Hospital (HCRP -No. 12407/09).
The volunteers were randomly allocated to five groups with 12 subjects each to receive different doses of CBD (100, 300, and 900 mg), clonazepam (1 mg) or placebo in a double-blind, randomized design. The groups were matched according to gender, age, body mass index (BMI), and STAI-trait score. One volunteer from the CBD-300 group could not attend the experimental session due to personal issues and was withdrawn from the study.
Volunteers began the experimental session after a minimum of 6 h of sleep and 2 h after eating a standard breakfast (200 ml chocolate milk and 100 g of bread with margarine).
The 12 volunteers sat in chairs arranged in a semicircle, following a predetermined order by the treatment number assigned to each one of them and performed the baseline measurements, followed by the intake of the five capsules within a 5-min interval for each volunteer. This phase was completed over a period of 1 h. The volunteers then watched a film about birds of Amazon forest lasting about 20 min. After that, the measurements were repeated with an interval of 5 min for each subject (pretest measure). 2 h and 20 min after the first volunteer had received the treatment, they all watched a video lasting about 10 min with recorded instructions for the speech test. 2 h and 30 min after the first volunteer received the treatment, they started performing the speech test, which lasted 5 min. Subsequently, the other volunteers were submitted to the speech test in the same order in which they had received the treatments. The speech test consisted of silently preparing a 1-min speech on a theme related to "the conditions of one public service of your city," chosen by chance from 12 options and revealed immediately before the speech preparation. The prepared speech was then...
Machine-readable layer
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