Ischemic Preconditioning
Objective: Assessment of VEGF-A level changes and retinal cell apoptosis reduction following 24-hour ischemic preconditioning before ischemia-reperfusion injury
This is a Ischemic Preconditioning protocol using Not explicitly stated in provided text as the model organism. The procedure involves 6 procedural steps. Extracted from a 2007 paper published in American Journal Of Pathology.
Model and subjects
Not explicitly stated in provided text • Not explicitly stated in provided text • unknown • Not explicitly stated in provided text • Not explicitly stated in provided text
Study window
~48 hours hands-on
Core workflow
Ischemic Preconditioning • Ischemia-Reperfusion Injury • Measure VEGF-A Levels
Primary readouts
- VEGF-A levels in retinal tissue
- Number of apoptotic retinal cells
- Retinal ganglion cell count
- Vascular parameters
Key equipment and reagents
Verified items
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Direct vendor links
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Protocol Steps
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Ischemic Preconditioning
Perform ischemic preconditioning 24 hours before ischemia-reperfusion injury
Note: This preconditioning step precedes the ischemia-reperfusion injury protocol
View evidence from paper
“Ischemic preconditioning 24 hours before ischemia-reperfusion injury increased VEGF-A levels”
Ischemia-Reperfusion Injury
Induce ischemia-reperfusion injury following the 24-hour preconditioning period
Note: Performed after ischemic preconditioning
View evidence from paper
“ischemia-reperfusion injury increased VEGF-A levels and substantially decreased the number of apoptotic retinal cells”
Measure VEGF-A Levels
Assess VEGF-A levels in retinal tissue following ischemic preconditioning and ischemia-reperfusion injury
Note: VEGF-A levels increased as part of adaptive response to ischemia
View evidence from paper
“Ischemic preconditioning 24 hours before ischemia-reperfusion injury increased VEGF-A levels”
Assess Retinal Cell Apoptosis
Quantify apoptotic retinal cells following ischemic preconditioning and ischemia-reperfusion injury
Note: Apoptotic cell count substantially decreased with ischemic preconditioning
View evidence from paper
“substantially decreased the number of apoptotic retinal cells”
VEGF-A Inhibition Study
Inhibit VEGF-A function and assess reversal of ischemic preconditioning protective effects
Note: Protective effect of ischemic preconditioning was reversed after VEGF-A inhibition
View evidence from paper
“The protective effect of ischemic preconditioning was reversed after VEGF-A inhibition”
Chronic VEGF-A Inhibition in Normal Animals
Chronically inhibit VEGF-A function in normal adult animals and assess retinal ganglion cell loss
Note: Chronic VEGF-A inhibition led to significant loss of retinal ganglion cells with no observable effect on vascular parameters
View evidence from paper
“chronic inhibition of VEGF-A function in normal adult animals led to a significant loss of retinal ganglion cells”