Lateral Pallium Ablation
Objective: To assess the role of the lateral telencephalic pallia (LP) in retention of conditioned avoidance responses in goldfish, comparing effects of LP ablation versus medial pallia (MP) ablation across nontrace and trace avoidance conditioning procedures
Protocol Steps
Nontrace Avoidance Conditioning Training
Fish were trained in nontrace avoidance conditioning procedure where the conditioned stimulus and unconditioned stimulus overlap temporally without a gap between them
Note: This is the first experimental condition used to establish baseline avoidance responses
View evidence from paper
“In the first experiment, fish were trained in nontrace avoidance conditioning.”
Trace Avoidance Conditioning Training
Fish were trained in trace avoidance conditioning procedure in which temporal cues were crucial for the learning process, with a temporal gap between the conditioned stimulus and unconditioned stimulus
Note: This is the second experimental condition where temporal information is essential
View evidence from paper
“In the second experiment, fish were trained in trace avoidance conditioning in which temporal cues were crucial for the learning process.”
Lateral Pallium (LP) Ablation Surgery
Surgical ablation of the lateral telencephalic pallia was performed on trained fish to assess its role in retention of the previously acquired conditioned avoidance response
Note: LP ablation was performed after training to test retention of learned responses
View evidence from paper
“Ablation of the lateral telencephalic pallia (LP) was performed to assess its role in retention of conditioned avoidance responses.”
Medial Pallium (MP) Ablation Surgery
Surgical ablation of the medial telencephalic pallia was performed on trained fish as a comparison condition to assess differential effects on avoidance retention
Note: MP ablation served as a comparison to LP ablation to determine differential memory system involvement
View evidence from paper
“The effects of ablation of the medial telencephalic pallia (MP) and lateral telencephalic pallia (LP) in goldfish on the retention of a conditioned avoidance response previously acquired in two experimental conditions.”
Retention Testing - Nontrace Condition
Fish with LP or MP ablations were tested for retention of the avoidance response in the nontrace avoidance conditioning procedure
Note: Testing occurred after ablation surgery to measure retention deficits
View evidence from paper
“An MP lesion affected the retention of the avoidance response in both procedures; in contrast, an LP lesion impaired the retention only in the trace-conditioning procedure.”
Retention Testing - Trace Condition
Fish with LP or MP ablations were tested for retention of the avoidance response in the trace avoidance conditioning procedure where temporal cues are crucial
Note: LP lesions specifically impaired retention in this temporal task
View evidence from paper
“An MP lesion affected the retention of the avoidance response in both procedures; in contrast, an LP lesion impaired the retention only in the trace-conditioning procedure.”