Medial Pallium Ablation
Objective: To assess the role of medial pallium (MP) ablation in retention of conditioned avoidance responses in goldfish, comparing effects between nontrace and trace avoidance conditioning procedures
This is a Medial Pallium Ablation protocol using goldfish as the model organism. The procedure involves 5 procedural steps. Extracted from a 2004 paper published in Journal of Neuroscience.
Model and subjects
goldfish
Study window
Estimated timing pending
Core workflow
Nontrace Avoidance Conditioning Training • Trace Avoidance Conditioning Training • Medial Pallium (MP) Ablation
Primary readouts
- Retention of conditioned avoidance response following MP ablation
- Retention of conditioned avoidance response following LP ablation
- Differential effects between nontrace and trace conditioning procedures
- Comparison of emotional memory system (MP) versus spatial/relational/temporal memory system (LP)
Key equipment and reagents
Verified items
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Direct vendor links
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Protocol Steps
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Nontrace Avoidance Conditioning Training
Fish were trained in nontrace avoidance conditioning procedure
Note: This was the first experimental condition used to establish baseline avoidance response
View evidence from paper
“In the first experiment, fish were trained in nontrace avoidance conditioning.”
Trace Avoidance Conditioning Training
Fish were trained in trace avoidance conditioning in which temporal cues were crucial for the learning process
Note: This was the second experimental condition where temporal cues were essential
View evidence from paper
“In the second experiment, fish were trained in trace avoidance conditioning in which temporal cues were crucial for the learning process.”
Medial Pallium (MP) Ablation
Ablation of the medial telencephalic pallia was performed on trained fish
Note: Surgical procedure to remove medial pallium tissue
View evidence from paper
“Ablation of the medial telencephalic pallia (MP) was performed to assess its role in retention of conditioned avoidance responses.”
Lateral Pallium (LP) Ablation
Ablation of the lateral telencephalic pallia was performed on trained fish as a control comparison
Note: Surgical procedure to remove lateral pallium tissue for comparative analysis
View evidence from paper
“effects of ablation of the medial telencephalic pallia (MP) and lateral telencephalic pallia (LP) in goldfish on the retention of a conditioned avoidance response”
Assessment of Avoidance Response Retention
Retention of the previously acquired conditioned avoidance response was tested following ablation in both nontrace and trace conditioning procedures
Note: Testing occurred after surgical ablation to measure effects on memory retention
View evidence from paper
“An MP lesion affected the retention of the avoidance response in both procedures; in contrast, an LP lesion impaired the retention only in the trace-conditioning procedure.”