Source Paper
Subventricular Zone-Derived Neuroblasts Migrate and Differentiate into Mature Neurons in the Post-Stroke Adult Striatum
T. Yamashita, M. Ninomiya, P. Hernandez Acosta, J. M. Garcia-Verdugo, T. Sunabori et al.
Journal of Neuroscience • 2006
Middle cerebral artery occlusion (MCAO)
Objective: To investigate whether subventricular zone (SVZ)-derived neuroblasts migrate to and differentiate into mature neurons in the striatum following middle cerebral artery occlusion (MCAO)-induced stroke
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Equipment1
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Protocol Steps
Induce stroke via middle cerebral artery occlusion
Perform middle cerebral artery occlusion (MCAO) surgery to induce stroke and trigger post-stroke neurogenesis in the striatum
Note: This procedure triggers the migration of SVZ-derived neuroblasts toward the injured striatum
View evidence from paper
“Stroke was induced via middle cerebral artery occlusion to trigger post-stroke neurogenesis in the striatum”
Label SVZ GFAP-expressing cells
Label GFAP-expressing cells in the subventricular zone using a cell-type-specific viral infection method to track their fate
Note: Cell-type-specific viral infection method used for labeling
View evidence from paper
“SVZ GFAP-expressing cells labeled by a cell-type-specific viral infection method were found to generate neuroblasts”
Observe neuroblast migration and chain formation
Monitor and document the migration of labeled neuroblasts toward the injured striatum and their formation of elongated chain-like cell aggregates
Note: Neuroblasts form chain-like aggregates similar to those in normal SVZ, closely associated with astrocytic processes and blood vessels
View evidence from paper
“These neuroblasts in the striatum formed elongated chain-like cell aggregates similar to those in the normal SVZ”
Long-term tracing of SVZ-derived neuroblasts
Use Cre-loxP system to perform long-term tracing of green fluorescent-labeled cells to determine their differentiation and maturation
Note: Cre-loxP genetic system used for cell lineage tracing
View evidence from paper
“long-term tracing of the green fluorescent-labeled cells with a Cre-loxP system revealed that the SVZ-derived neuroblasts differentiated”
Assess neuronal differentiation and maturation
Evaluate whether SVZ-derived neuroblasts have differentiated into mature neurons by examining expression of neuronal-specific nuclear protein and synapse formation
Note: Mature neurons express neuronal-specific nuclear protein and form synapses with neighboring striatal cells
View evidence from paper
“the SVZ-derived neuroblasts differentiated into mature neurons in the striatum, in which they expressed neuronal-specific nuclear protein and formed synapses”