Morphine Conditioned Place Preference
Objective: To test whether an established morphine conditioned place preference (mCPP) can be persistently disrupted by inhibiting protein synthesis after reactivation of the conditioning memory trace
This is a Morphine Conditioned Place Preference protocol using Not explicitly stated in provided text as the model organism. The procedure involves 5 procedural steps, 1 equipment items, 3 materials. Extracted from a 2006 paper published in Journal of Neuroscience.
Model and subjects
Not explicitly stated in provided text • Not explicitly stated in provided text • unknown • Not explicitly stated in provided text • Not explicitly stated in provided text
Study window
Estimated timing pending
Core workflow
Establish morphine conditioned place preference • Reactivate conditioning memory • Administer protein synthesis inhibitor
Primary readouts
- Morphine conditioned place preference score
- Time spent in drug-paired context
- Persistence of preference disruption after further conditioning
- Regional specificity of protein synthesis inhibition effects
Key equipment and reagents
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Protocol Steps
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Establish morphine conditioned place preference
Condition animals with morphine in a specific context to establish a conditioned place preference
Note: This creates the baseline established mCPP that will be tested for disruption
View evidence from paper
“an established morphine conditioned place preference (mCPP) was persistently disrupted if protein synthesis was blocked”
Reactivate conditioning memory
Reactivate the established morphine conditioning memory by re-exposing animals to both the conditioning context and the drug concomitantly
Note: Unlike contextual recall alone, an established mCPP required concomitant re-experience of both context and drug for memory lability
View evidence from paper
“an established mCPP did not become labile after contextual recall, but required the concomitant re-experience of both the conditioning context and the drug”
Administer protein synthesis inhibitor
Block protein synthesis using either anisomycin or cycloheximide after the representation of the conditioning session
Note: Administration must occur after memory reactivation to disrupt reconsolidation
View evidence from paper
“an established morphine conditioned place preference (mCPP) was persistently disrupted if protein synthesis was blocked by either anisomycin or cycloheximide after the representation of a conditioning session”
Test regional specificity of disruption
Selectively block protein synthesis in specific brain regions: hippocampus, basolateral amygdala, nucleus accumbens, or ventral tegmental area
Note: Disruption occurred in hippocampus, basolateral amygdala, and nucleus accumbens but not in ventral tegmental area
View evidence from paper
“an established mCPP was disrupted after the conditioning session if protein synthesis was blocked selectively in the hippocampus, basolateral amygdala, or nucleus accumbens but not in the ventral tegmental area”
Assess persistence of disruption
Test whether the disruption of mCPP is permanent by attempting further conditioning
Note: The preference did not return after further conditioning, indicating permanent disruption
View evidence from paper
“This disruption seems to be permanent, because the preference did not return after further conditioning”