Source Paper
Tubuloside B ameliorates blood-brain barrier disruption and cognitive deficits after cerebral ischemia by inhibiting TRIC protein ubiquitination and degradation
He M, Zhang H, Liu R, Zhang C, Li J et al.
J Neuroinflammation • 2025
Morris Water Maze
Objective: To assess spatial learning and memory abilities using a water maze behavioral test
This is a Morris Water Maze protocol using Rat as the model organism. The procedure involves 4 procedural steps, 1 materials. Extracted from a 2025 paper published in J Neuroinflammation.
Model and subjects
Rat • Sprague-Dawley • Male • 8-10 weeks • 40
Study window
Estimated timing pending
Core workflow
Training phase • Probe test • Data recording
Primary readouts
- Escape latency
- Path length
- Time in target quadrant
Key equipment and reagents
Verified items
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Direct vendor links
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Protocol Steps
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Training phase
Conduct spatial learning training over 5 consecutive days with hidden platform
View evidence from paper
“Spatial learning and memory were assessed over 5 training days with a hidden platform”
Probe test
Perform probe test on day 6 following training
View evidence from paper
“A probe test was conducted on day 6”
Data recording
Record escape latency, path length, and time spent in target quadrant
View evidence from paper
“Escape latency, path length, and time in the target quadrant were recorded”
Blinded analysis
Perform behavioral tests and data analysis by blinded investigators
View evidence from paper
“Behavioral tests and data analysis were performed by investigators blinded to the group assignments”