Source Paper
Neuroprotective Effects of Alpha-Lipoic Acid Against Behavioral Toxicity, Oxidative and Inflammatory Damage Caused by Titanium Dioxide Nanoparticles
Khedr MA, El-Kazaz SE, Rashed RR, Tohamy HG, Shukry M et al.
Biol Trace Elem Res • 2025
Morris Water Maze
Objective: Analyze spatial learning and memory using the Morris Water Maze
This is a Morris Water Maze protocol using Rat as the model organism. The procedure involves 4 procedural steps, 1 equipment items, 2 materials. Extracted from a 2025 paper published in Biol Trace Elem Res.
Model and subjects
Rat • Sprague-Dawley • male • 3- to 4-month-old • 24
Study window
Estimated timing pending
Core workflow
Tank setup • Place navigation phase (Day 1) • Trial termination criteria
Primary readouts
- Time passed to locate the platform (escape)
- Time needed to reach the target quadrant
- Time spent in the target quadrant
- Number of trials to reach the target quadrant
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Tank setup
Fill spherical tank with water to 45 cm depth at 23±1°C, add brown dye, divide into 4 quadrants with hidden platform in quadrant 3
View evidence from paper
“water filling to 45 cm depth maintained at 23 ± 1 °C”
Place navigation phase (Day 1)
Each rat undergoes 4 trials starting from different quadrants in clockwise sequence, platform position fixed in target quadrant
View evidence from paper
“Each rat was liberated into the water facing the tank wall and underwent four trials starting from a different quadrant in a clockwise sequence”
Trial termination criteria
Each trial ends when rat reaches platform or after 60 seconds; if not found, guide rat to platform for 5-10 seconds
View evidence from paper
“Each trial terminated when the rat reached the platform or after 60 s”
Spatial probe phase (Day 2)
Remove platform, release rat from target quadrant wall, allow swimming for 60 seconds
View evidence from paper
“The platform was removed in the spatial probe phase (second day)”