Morris Water Maze
Objective: Assessment of hippocampal-dependent spatial learning and memory using the Morris water maze task
This is a Morris Water Maze protocol using Not explicitly stated in provided text as the model organism. The procedure involves 6 procedural steps, 2 equipment items. Extracted from a 2006 paper published in Proceedings of the National Academy of Sciences.
Model and subjects
Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text • Not explicitly stated in provided text
Study window
Estimated timing pending
Core workflow
Hippocampal Neurogenesis Ablation - Method 1 • Hippocampal Neurogenesis Ablation - Method 2 • Morris Water Maze Testing
Primary readouts
- Spatial learning performance in Morris water maze
- Spatial learning performance in Y maze
- Contextual fear conditioning response
- Cued conditioning response
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Hippocampal Neurogenesis Ablation - Method 1
Focal X irradiation of the hippocampus to ablate neurogenesis
Note: One of two independent methods used to ablate hippocampal neurogenesis
View evidence from paper
“focal X irradiation of the hippocampus or genetic ablation of glial fibrillary acidic protein-positive neural progenitor cells”
Hippocampal Neurogenesis Ablation - Method 2
Genetic ablation of glial fibrillary acidic protein-positive neural progenitor cells
Note: One of two independent methods used to ablate hippocampal neurogenesis
View evidence from paper
“focal X irradiation of the hippocampus or genetic ablation of glial fibrillary acidic protein-positive neural progenitor cells”
Morris Water Maze Testing
Assessment of hippocampal-dependent spatial learning and memory
Note: Task was unaffected by neurogenesis ablation procedures
View evidence from paper
“Hippocampal-dependent spatial learning tasks such as the Morris water maze and Y maze were unaffected.”
Y Maze Testing
Assessment of hippocampal-dependent spatial learning and memory
Note: Task was unaffected by neurogenesis ablation procedures
View evidence from paper
“Hippocampal-dependent spatial learning tasks such as the Morris water maze and Y maze were unaffected.”
Contextual Fear Conditioning
Assessment of hippocampal-dependent contextual learning
Note: This task was impaired by neurogenesis ablation procedures
View evidence from paper
“each procedure caused a limited behavioral deficit and a loss of synaptic plasticity within the dentate gyrus. Specifically, focal X irradiation of the hippocampus or genetic ablation of glial fibrillary acidic protein-positive neural progenitor cells impaired contextual fear conditioning”
Cued Conditioning
Assessment of non-hippocampal dependent conditioning
Note: This task was not impaired by neurogenesis ablation procedures
View evidence from paper
“impaired contextual fear conditioning but not cued conditioning”