Morris Water Maze
Objective: Assessment of hippocampal-dependent spatial learning and memory using the Morris water maze task
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Equipment2
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Protocol Steps
Hippocampal Neurogenesis Ablation - Method 1
Focal X irradiation of the hippocampus to ablate neurogenesis
Note: One of two independent methods used to ablate hippocampal neurogenesis
View evidence from paper
“focal X irradiation of the hippocampus or genetic ablation of glial fibrillary acidic protein-positive neural progenitor cells”
Hippocampal Neurogenesis Ablation - Method 2
Genetic ablation of glial fibrillary acidic protein-positive neural progenitor cells
Note: One of two independent methods used to ablate hippocampal neurogenesis
View evidence from paper
“focal X irradiation of the hippocampus or genetic ablation of glial fibrillary acidic protein-positive neural progenitor cells”
Morris Water Maze Testing
Assessment of hippocampal-dependent spatial learning and memory
Note: Task was unaffected by neurogenesis ablation procedures
View evidence from paper
“Hippocampal-dependent spatial learning tasks such as the Morris water maze and Y maze were unaffected.”
Y Maze Testing
Assessment of hippocampal-dependent spatial learning and memory
Note: Task was unaffected by neurogenesis ablation procedures
View evidence from paper
“Hippocampal-dependent spatial learning tasks such as the Morris water maze and Y maze were unaffected.”
Contextual Fear Conditioning
Assessment of hippocampal-dependent contextual learning
Note: This task was impaired by neurogenesis ablation procedures
View evidence from paper
“each procedure caused a limited behavioral deficit and a loss of synaptic plasticity within the dentate gyrus. Specifically, focal X irradiation of the hippocampus or genetic ablation of glial fibrillary acidic protein-positive neural progenitor cells impaired contextual fear conditioning”
Cued Conditioning
Assessment of non-hippocampal dependent conditioning
Note: This task was not impaired by neurogenesis ablation procedures
View evidence from paper
“impaired contextual fear conditioning but not cued conditioning”