Motor Coordination Test
Objective: Assessment of motor coordination to measure motor recovery following experimental permanent stroke
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Protocol Steps
Surgical Procedure - Permanent Middle Cerebral Artery Occlusion
Male Sprague-Dawley rats (12 months old) underwent permanent middle cerebral artery occlusion or sham operations
Note: Sham operations served as control group
View evidence from paper
“We tested male Sprague-Dawley rats (12 months old) with permanent middle cerebral artery occlusion or sham operations”
Dose-Response Study - Initial Progesterone Administration
Intraperitoneal injections of progesterone administered at 1 hour post-occlusion
Note: Three dose levels tested: 8, 16, or 32 mg/kg
View evidence from paper
“animals received intraperitoneal injections of progesterone (8, 16 or 32 mg/kg) at 1 h post-occlusion”
Dose-Response Study - Subcutaneous Injections
Subcutaneous injections of progesterone administered at 6 hours post-occlusion and then once every 24 hours for 7 days
Note: Same dose levels as intraperitoneal injections
View evidence from paper
“subcutaneous injections at 6 h and then once every 24 h for 7 days”
Time-Window Study - Progesterone Administration
Optimal dose of progesterone administered starting at 3, 6, or 24 hours post-stroke
Note: The optimal dose was determined from the dose-response study
View evidence from paper
“For the time-window study, the optimal dose of progesterone was given starting at 3, 6 or 24 h post-stroke”
Behavioral Testing - Motor Coordination Assessment
Motor coordination testing performed as part of comprehensive behavioral recovery evaluation
Note: Part of multiple measures including sensory, motor and cognitive performance
View evidence from paper
“improved functional outcomes up to 3 weeks after stroke on locomotor activity, grip strength, sensory neglect, gait impairment, motor coordination and spatial navigation tests”
Brain Extraction and Infarct Volume Evaluation
Rats were killed and brains extracted for evaluation of infarct volume
Note: Terminal timepoint for histological analysis
View evidence from paper
“Rats were killed at 22 days post-stroke and brains extracted for evaluation of infarct volume”